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PMID: 6597338 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The sup8 tRNALeu gene of Schizosaccharomyces pombe has an unusual intervening sequence and reduced pairing in the anticodon stem.

Molecular & general genetics : MGG ·Vol. 197 ·No. 3 ·1984-00-00 ·Pages 447-52

Sumner-Smith M, Hottinger H, Willis I, Koch TL, Arentzen R, Söll D

Abstract

We have cloned and sequenced the wild-type and suppressor alleles of the S. pombe sup8 tRNA gene. The wild-type allele has a leucine UAA anticodon and the suppressor (sup8-e) carries the opal suppressor anticodon UCA. The gene has a 16 base pair intervening sequence that, in the RNA, is predicted to form a secondary structure which involves base pairing to the 5', rather than the usual 3' side of the 5' splice site. When incubated in Saccharomyces cerevisiae cell-free extracts both alleles are efficiently transcribed, the 5' leader and 3' trailer sequences are removed and CCA is added to the 3' processed end; however, the intervening sequence is not excised. This finding implies that the structural requirements of the splicing endonucleases in the two yeasts have diverged. No other tRNA genes with related sequences were detected in S. pombe DNA by hybridization, suggesting that other UUA isoacceptors may be structurally dissimilar to sup8 or that the UUA codon may be decoded by a UUG leucine isoacceptor.

MeSH Terms
Alleles Anticodon Ascomycota/genetics Base Sequence Cloning, Molecular Genes, Fungal Hydrogen Bonding Leucine Nucleic Acid Conformation RNA, Transfer/genetics Schizosaccharomyces/genetics Suppression, Genetic
Chemicals
Anticodon RNA, Transfer Leucine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sumner-Smith M
Hottinger H
Willis I
Koch T L
Arentzen R
Söll D
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00329941
Published
1984-00-00
Pages
447-52
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X01181
External Links
PubMed source DOI: 10.1007/BF00329941
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