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PMID: 659637 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The activation of plasminogen by Hageman factor (Factor XII) and Hageman factor fragments.

The Journal of clinical investigation ·Vol. 62 ·No. 1 ·1978-07-00 ·Pages 54-60

Goldsmith GH, Saito H, Ratnoff OS

Abstract

Activation of plasminogen through surface-mediated reactions is well recognized. In the presence of kaolin, purified Hageman factor (Factor XII) changed plasminogen to plasmin, as assayed upon a synthetic amide substrate and by fibrinolysis. Kinetic studies suggested an enzymatic action of Hageman factor upon its substrate, plasminogen. Hageman factor fragments, at a protein concentration equivalent to whole Hageman factor, activated plasminogen to a lesser extent. These protein preparations were not contaminated with other agents implicated in surface-mediated fibrinolysis. Diisopropyl fluorophosphate treatment of plasminogen did not inhibit its activation by Hageman factor. These studies indicate that Hageman factor has a hitherto unsuspected function, the direct activation of plasminogen.

MeSH Terms
Amides/blood Factor XII/analogs & derivatives,antagonists & inhibitors,pharmacology Fibrinolysis Humans In Vitro Techniques Peptide Fragments/pharmacology Plasminogen Activators
Chemicals
Amides Peptide Fragments Factor XII Plasminogen Activators
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Goldsmith G H
Saito H
Ratnoff O S
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21 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI109113
Published
1978-07-00
Pages
54-60
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC371736
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI109113 PMC Full Text View journal details
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