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PMID: 6589620 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and primary sequence of an unspliced human urokinase poly(A)+ RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 15 ·1984-08-00 ·Pages 4727-31

Verde P, Stoppelli MP, Galeffi P, Di Nocera P, Blasi F

Abstract

Human urokinase cDNA clones have been identified from a cDNA library prepared from total RNA of human fibroblasts transformed by simian virus 40 [Okayama, H. & Berg, P. (1983) Mol. Cell. Biol. 3, 280-289]. Synthetic oligonucleotides, corresponding to urokinase protein sequence, were used as probes. The cloned cDNA covers most of the coding sequence and the entire 3' untranslated region. The nucleotide sequence of one of the clones identifies this as a copy of a partially spliced polyadenylylated precursor to urokinase mRNA. The introns separate functionally different domains of the enzyme. Human urokinase mRNA has been identified by RNA blot and its size was estimated at 2500 nucleotides.

MeSH Terms
Base Sequence Cells, Cultured DNA/genetics Humans Nucleic Acid Precursors/isolation & purification RNA Splicing RNA, Messenger/genetics,isolation & purification Urokinase-Type Plasminogen Activator/genetics
Chemicals
Nucleic Acid Precursors RNA, Messenger DNA Urokinase-Type Plasminogen Activator
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Verde P
Stoppelli M P
Galeffi P
Di Nocera P
Blasi F
References (30)
30 references, click to expand
  1. The role of proteinases in cellular invasiveness.
    Biochim Biophys Acta. 1983 Dec 29;695(3-4):177-214 PMID: 6317025
  2. Antibodies to plasminogen activator inhibit human tumor metastasis.
    Cell. 1983 Dec;35(3 Pt 2):611-9 PMID: 6418388
  3. Monoclonal antibodies to human urokinase identify the single-chain pro-urokinase precursor.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):110-4 PMID: 6364130
  4. High efficiency of replication and expression of foreign genes in SV40-transformed human fibroblasts.
    EMBO J. 1983;2(12):2327-31 PMID: 6321161
  5. The isolation and characterization of plasminogen activators (urokinase) from human urine.
    Biochemistry. 1966 Jul;5(7):2160-9 PMID: 4959959
  6. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  7. An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. I. Chick embryo fibroblast cultures transformed by avian RNA tumor viruses.
    J Exp Med. 1973 Jan 1;137(1):85-111 PMID: 4347290
  8. Structure and specific binding of trypsin: comparison of inhibited derivatives and a model for substrate binding.
    J Mol Biol. 1974 Feb 25;83(2):209-30 PMID: 4821871
  9. Induction of macrophage plasminogen activator by endotoxin stimulation and phagocytosis: evidence for a two-stage process.
    J Exp Med. 1974 Oct 1;140(4):995-1010 PMID: 4427092
  10. Tumour promotor induces plasminogen activator.
    Nature. 1976 Jan 22;259(5540):232-3 PMID: 1250353
  11. Nerve growth factor receptors on human melanoma cells in culture.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):565-9 PMID: 265522
  12. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  13. Primary activation of the vitellogenin gene in the rooster.
    J Biol Chem. 1977 Nov 25;252(22):8310-9 PMID: 914873
  14. Epidermal growth factor, like phorbol esters, induces plasminogen activator in HeLa cells.
    Nature. 1978 Aug 17;274(5672):696-7 PMID: 307695
  15. A ubiquitous family of repeated DNA sequences in the human genome.
    J Mol Biol. 1979 Aug 15;132(3):289-306 PMID: 533893
  16. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  17. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  18. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
  19. Regulation of plasminogen activator synthesis in chick embryo fibroblasts infected with avian retroviruses.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:665-8 PMID: 6253167
  20. Plasminogen activator is an apparent lymphocyte mitogen.
    J Immunol. 1981 Apr;126(4):1415-20 PMID: 6259259
  21. Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.
    Nucleic Acids Res. 1981 Apr 10;9(7):1691-706 PMID: 7232224
  22. Structural relationship between human high and low molecular mass urokinase.
    Hoppe Seylers Z Physiol Chem. 1982 Feb;363(2):133-41 PMID: 7037592
  23. A proenzyme form of human urokinase.
    J Biol Chem. 1982 Jun 25;257(12):7262-8 PMID: 6806270
  24. The primary structure of high molecular mass urokinase from human urine. The complete amino acid sequence of the A chain.
    Hoppe Seylers Z Physiol Chem. 1982 Oct;363(10):1155-65 PMID: 6754569
  25. The complete amino acid sequence of low molecular mass urokinase from human urine.
    Hoppe Seylers Z Physiol Chem. 1982 Sep;363(9):1043-58 PMID: 6754572
  26. Purification of zymogen to plasminogen activator from human glioblastoma cells by affinity chromatography with monoclonal antibody.
    Biochemistry. 1982 Dec 7;21(25):6410-5 PMID: 6891264
  27. Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli.
    Nature. 1983 Jan 20;301(5897):214-21 PMID: 6337343
  28. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
    Mol Cell Biol. 1983 Feb;3(2):280-9 PMID: 6300662
  29. Hormonal regulation of plasminogen activator mRNA production in porcine kidney cells.
    Cell. 1983 Apr;32(4):1181-90 PMID: 6687701
  30. Structure of mouse kallikrein gene family suggests a role in specific processing of biologically active peptides.
    Nature. 1983 May 26;303(5915):300-7 PMID: 6602295
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.15.4727
Published
1984-08-00
Pages
4727-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391563
Subset
IM
Databases
GENBANK
D00244, K02286
External Links
PubMed source DOI: 10.1073/pnas.81.15.4727 PMC Full Text View journal details
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