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PMID: 6578503 Published · ppublish English Journal Article

Replication and expression of Xenopus laevis globin genes injected into fertilized Xenopus eggs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 20 ·1983-10-00 ·Pages 6197-201

Bendig MM, Williams JG

Abstract

Cloned Xenopus adult alpha 1- and beta 1-globin genes were injected into fertilized Xenopus eggs, and the eggs were allowed to develop into swimming tadpoles. The injected DNA replicated during early Xenopus development but did not become methylated de novo. When DNA was modified with Hpa II methylase before injection, methylation was maintained during replication. Although the Xenopus adult globin genes are not normally expressed until metamorphosis, both the unmethylated and in vitro methylated adult alpha- and beta-globin genes were transcribed at low levels, but from the correct promoters, during early development.

MeSH Terms
Animals DNA Replication Female Gene Expression Regulation Genes Globins/genetics Methylation Transcription, Genetic Xenopus laevis/genetics Zygote/physiology
Chemicals
Globins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bendig M M
Williams J G
References (21)
21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.20.6197
Published
1983-10-00
Pages
6197-201
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394262
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.20.6197 PMC Full Text View journal details
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