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PMID: 6547670 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence of a complete chicken delta-crystallin cDNA.

The EMBO journal ·Vol. 3 ·No. 6 ·1984-06-00 ·Pages 1397-402

Yasuda K, Nakajima N, Isobe T, Okada TS, Shimura Y

Abstract

The nucleotide sequence of a full length cDNA of delta-crystallin mRNA from chicken lens has been determined using a delta-crystallin cDNA clone (pB delta 11), which represents the mRNA sequence of 1530 nucleotides from the poly(A) junction but does not contain the 5'-terminal sequence of 44 nucleotides of the mRNA. The 5'-terminal sequence of the mRNA, absent in the cDNA clone, has been determined with a stretch of cDNA sequence by the primer extension procedure. The amino acid sequence deduced from the nucleotide sequence is consistent with the amino acid sequences of several tryptic peptides, the total amino acid composition, and the mol. wt. of delta-crystallin estimated by SDS-polyacrylamide gel electrophoresis. The computer-assisted analysis predicts high alpha-helical content throughout the polypeptide. Sequence analyses have revealed that gene 1 encodes the mRNA from which the cDNA clone was derived.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chickens Cloning, Molecular Crystallins/genetics DNA/analysis Peptide Fragments/analysis RNA, Messenger/genetics Trypsin
Chemicals
Crystallins Peptide Fragments RNA, Messenger DNA Trypsin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yasuda K
Nakajima N
Isobe T
Okada T S
Shimura Y
References (31)
31 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-06-00
Pages
1397-402
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557529
Subset
IM
Databases
GENBANK
K03257, N00025, X00626
External Links
PubMed source PMC Full Text View journal details
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