Home LiteratureArticle Details
PMID: 6547530 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

31P-NMR analysis of the B to Z transition in double-stranded (dC-dG)3 and (dC-dG)4 in high salt solution.

Nucleic acids research ·Vol. 12 ·No. 11 ·1984-06-11 ·Pages 4625-35

Holak TA, Borer PN, Levy GC, van Boom JH, Wang AH

Abstract

In 4M NaCl solutions (dC-dG)n (n = 3,4; approximately 9 mM) exist as a mixture o +/- B and Z forms. The low and high field components of two 31P NMR resonances originating from internal phosphodiester groups are assigned to the GpC and CpG linkages, respectively. Low temperatures stabilize the Z-forms, which completely disappear above 50 degrees C (n = 3) and 65 degrees C (n = 4). delta H = -44 and -17 kJ/mol for B to Z transition in the hexamer and octamer duplexes, respectively. Temperature dependent changes (0-50 degrees C range) in the spin-lattice relaxation times at 145.7 MHz are distinctly different for the 31P nuclei o +/- GpC and CpG groups. The relaxation data can be explained by assuming that the GpC phosphodiester groups undergo more local internal motion than do the CpG groups.

MeSH Terms
Calorimetry Magnetic Resonance Spectroscopy/methods Nucleic Acid Conformation Oligodeoxyribonucleotides Oligonucleotides Osmolar Concentration Structure-Activity Relationship
Chemicals
Oligodeoxyribonucleotides Oligonucleotides tri(deoxycytidylic acid-deoxyguanylic acid) tetra(deoxycytidylic acid-deoxyguanylic acid)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Holak T A
Borer P N
Levy G C
van Boom J H
Wang A H
References (27)
27 references, click to expand
  1. Optimised parameters for A-DNA and B-DNA.
    Biochem Biophys Res Commun. 1972 Jun 28;47(6):1504-9 PMID: 5040245
  2. 31P Magnetic relaxation studies of yeast transfer RNAPhe.
    Biopolymers. 1977 Mar;16(3):655-67 PMID: 321043
  3. Helix-coil transition of the self-complementary dG-dG-dA-dA-dT-dT-dC-dC duplex.
    Eur J Biochem. 1979 May 15;96(2):267-76 PMID: 456372
  4. "Alternating B-DNA" conformation for the oligo(dG-dC) duplex in high-salt solution.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2508-11 PMID: 288040
  5. An alternating conformation characterizes the phosphodiester backbone of poly(dA-dT) in solution.
    J Biol Chem. 1979 Sep 10;254(17):8125-8 PMID: 468810
  6. Conformation of 145 base pair length poly (dG-dC) . poly (dG-dC) in solution and in association with histones.
    Nucleic Acids Res. 1980 May 10;8(9):2093-103 PMID: 7433135
  7. Left-handed double helical DNA: variations in the backbone conformation.
    Science. 1981 Jan 9;211(4478):171-6 PMID: 7444458
  8. Characterization of alternating deoxyribonucleic acid conformations in solution by phosphorus-31 nuclear magnetic resonance spectroscopy.
    Biochemistry. 1981 May 26;20(11):3049-55 PMID: 7248266
  9. Nucleotide conformational analysis by 31P nuclear magnetic resonance spectroscopy.
    Annu Rev Biophys Bioeng. 1981;10:355-86 PMID: 7020579
  10. Strange double helix of poly (dA-dT) in high-salt solution.
    Biochem Biophys Res Commun. 1981 Apr 30;99(4):1257-64 PMID: 7259778
  11. Conformational stability of alternating d (CG) oligomers in high salt solution.
    Nucleic Acids Res. 1981 May 11;9(9):2195-206 PMID: 6272229
  12. Phosphorus-31 nuclear magnetic resonance of double- and triple-helical nucleic acids. Phosphorus-31 chemical shifts as a probe of phosphorus-oxygen ester bond torsional angles.
    Biochemistry. 1982 Feb 2;21(3):580-9 PMID: 6175342
  13. An effective method for the assignment of 31P-NMR resonance(s) of oligonucleotides.
    Biopolymers. 1982 Mar;21(3):697-701 PMID: 7066479
  14. Right-handed and left-handed DNA: studies of B- and Z-DNA by using proton nuclear Overhauser effect and P NMR.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1413-7 PMID: 6951185
  15. NMR relaxation in DNA. I. The contribution of torsional deformation modes of the elastic filament.
    Biopolymers. 1982 Apr;21(4):729-62 PMID: 7082764
  16. Spontaneous ordering of DNA. Effects of intermolecular interactions on DNA motional dynamics monitored by 13C and 31P nuclear magnetic resonance spectroscopy.
    J Biol Chem. 1983 Jan 10;258(1):250-6 PMID: 6848500
  17. Salt- and sequence-dependence of the secondary structure of DNA in solution by 31P-NMR spectroscopy.
    Biopolymers. 1983 Mar;22(3):879-93 PMID: 6850053
  18. B to Z transition of double-stranded poly[deoxyguanylyl(3'-5')-5-methyldeoxycytidine] in solution by phosphorus-31 and carbon-13 nuclear magnetic resonance spectroscopy.
    Biochemistry. 1983 Apr 26;22(9):2136-42 PMID: 6860654
  19. Generation of left-handed Z-DNA in solution and visualization in polytene chromosomes by immunofluorescence.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:143-54 PMID: 6345056
  20. Left-handed helical conformation of poly[d(A-m5C).d(G-T)].
    Nature. 1983 Jul 7-13;304(5921):83-6 PMID: 6866095
  21. Spectroscopic studies of (m5dC-dG)3: thermal stability of B- and Z-forms.
    Nucleic Acids Res. 1983 Jul 11;11(13):4453-66 PMID: 6866770
  22. Absorption of sulfate from orally administered magnesium sulfate in man.
    J Toxicol Clin Toxicol. 1983 Apr;20(2):107-14 PMID: 6887305
  23. Assignment of resonances in the phosphorus-31 nuclear magnetic resonance spectrum of poly[d(A-T)] from phosphorothioate substitution.
    Biochemistry. 1983 Sep 13;22(19):4546-50 PMID: 6354262
  24. Bromination stabilizes poly(dG-dC) in the Z-DNA form under low-salt conditions.
    Biochemistry. 1984 Jan 3;23(1):54-62 PMID: 6691966
  25. A one- and two-dimensional NMR study of the B to Z transition of (m5dC-dG)3 in methanolic solution.
    Nucleic Acids Res. 1984 Jan 25;12(2):1243-63 PMID: 6694910
  26. A critical evaluation of models for complex molecular dynamics: application of NMR studies of double- and single-stranded DNA.
    Biopolymers. 1983 Dec;22(12):2703-26 PMID: 6667336
  27. Understanding DNA conformational dynamics: answering questions and questioning answers.
    J Biomol Struct Dyn. 1983 Dec;1(3):795-808 PMID: 6400901
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.11.4625
Published
1984-06-11
Pages
4625-35
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318863
Subset
IM
Grants
NIGMS NIH HHS · GM29778 · United States
NIGMS NIH HHS · GM32691 · United States
NCRR NIH HHS · RR01317 · United States
External Links
PubMed source DOI: 10.1093/nar/12.11.4625 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2