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PMID: 6546794 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequence of human adenosine deaminase cDNA including the coding region and a small intron.

Nucleic acids research ·Vol. 12 ·No. 5 ·1984-03-12 ·Pages 2439-46

Wiginton DA, Adrian GS, Hutton JJ

Abstract

The nucleotide sequence for an unusual, cloned human adenosine deaminase cDNA has been determined. Contained within a sequence of 1535 nucleotides is a coding sequence of 1089 nucleotides that encodes a protein of 40,762 daltons. The coding sequence is interrupted by a non-coding region containing 76 nucleotides. Both the 3' and 5' ends of this region have consensus sequences generally associated with splice sites. The 3' untranslated sequence contained 308 nucleotides, including a polyadenylation signal sequence 20 nucleotides from the end. The cloned cDNA appears to correspond to a nuclear mRNA precursor which contains a small intron.

MeSH Terms
Adenosine Deaminase/genetics Amino Acid Sequence Base Sequence Cloning, Molecular Codon/genetics DNA/isolation & purification Genes Genetic Variation Humans Molecular Weight Nucleoside Deaminases/genetics
Chemicals
Codon DNA Nucleoside Deaminases Adenosine Deaminase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wiginton D A
Adrian G S
Hutton J J
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.5.2439
Published
1984-03-12
Pages
2439-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318674
Subset
IM
Grants
NICHD NIH HHS · HD-15036 · United States
Databases
GENBANK
X00427
External Links
PubMed source DOI: 10.1093/nar/12.5.2439 PMC Full Text View journal details
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