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PMID: 6528987 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Rapid precision interferometry for the analytical ultracentrifuge. III. Determination of period of rotation, frequency of rotation, and elapsed time.

Analytical biochemistry ·Vol. 143 ·No. 1 ·1984-11-15 ·Pages 103-12

Laue TM, Yphantis DA, Rhodes DG

Abstract

The approaches presented in this series of papers make possible rapid gathering, reduction, and analysis of data from the Rayleigh interference optical system of an analytical ultracentrifuge. Instrumentation described in this paper provides some of the timing and measurement circuits necessary for a microprocessor or minicomputer to determine the rotor frequency, rotor period, and elapsed time of an experiment. It includes simple but effective circuits to generate precise rotor timing pulses that are useful for synchronization of pulsed light sources. Circuits to control photographic operations in the ultracentrifuge are described briefly. All of these circuits are interfaced to a simple microcomputer address/data bus. An adapter between this bus and a Q-bus (for a DEC LSI-11/2 or LSI 11/23 microcomputer) is also described. The circuits presented have been used in this laboratory over a 3-year period. They have proven reliable and form an integral part of the real-time data acquisition systems that have been constructed.

MeSH Terms
Interferometry/instrumentation Minicomputers Rotation Time Factors Ultracentrifugation/instrumentation
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Laue T M
Yphantis D A
Rhodes D G
Article Info
Journal
Analytical biochemistry
Abbr.
Anal Biochem
ISSN
0003-2697
Published
1984-11-15
Pages
103-12
Language
English
Region
United States
NLM ID
0370535
Subset
IM
Grants
NIADDK NIH HHS · AM 18001 · United States
NIGMS NIH HHS · GM 00317 · United States
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