The Giemsa-11 technique for species-specific chromosome differentiation. A simple stain modification leading to dependable direct and sequential staining procedures.

Histochemistry ·Vol. 81 ·No. 5 ·1984-00-00 ·Pages 465-8

Buys CH, Aanstoot GH, Nienhaus AJ

Abstract

Oxidizing Methylene Blue and adding the reaction products to Eosin Y and Azure B makes possible a highly reliable Giemsa-11 technique for discrimination of chromosomes in hybrid cells according to their parental origin. This staining can be combined in a sequential procedure with a fluorescent banding technique allowing the exact identification of the chromosomes.

MeSH Terms

Animals Azure Stains Chromosomes/ultrastructure Chromosomes, Human/ultrastructure Cricetinae Cricetulus Humans Hybrid Cells/cytology Karyotyping Metaphase Mice Species Specificity

Chemicals

Azure Stains

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Buys C H

Aanstoot G H

Nienhaus A J

References (10)

10 references, click to expand

Differential staining of human and mouse chromosomes in interspecific cell hybrids.
Nature. 1974 Sep 6;251(5470):77-9 PMID: 4137611
Somatic cell genetics and gene families.
Science. 1983 May 27;220(4600):919-24 PMID: 6573776
Somatic cell genetics and flow cytometry.
Cytometry. 1983 Sep;4(2):99-108 PMID: 6354642
Simultaneous identification of chromatid replication and of human chromosomes in metaphases of man-mouse somatic cell hybrids. (With 1 color plate).
Cytogenet Cell Genet. 1977;19(4):236-9 PMID: 598253
Staining of some specific regions of human chromosomes, particularly the secondary constriction of No. 9.
Nat New Biol. 1972 Jul 26;238(82):122-4 PMID: 4114702
Mechanisms involved in the banding of chromosomes with guinacrine and Giems. II. The interaction of the dyes with the chromosomal components.
Exp Cell Res. 1973 Sep;81(1):223-6 PMID: 4127952
New approaches to the study of chromosome organization.
Sci Prog. 1983 Winter;68(272):543-64 PMID: 6367034
Reverse fluorescent chromosome banding with chromomycin and DAPI.
Chromosoma. 1976 Nov 29;58(4):307-24 PMID: 137107
Somatic cell genetics and gene mapping.
Int Rev Cytol. 1983;85:109-146 PMID: 6662657
Specific cytological recognition of the heterochromatic segment of number 9 chromosome in man.
Exp Cell Res. 1972 Jul;73(1):239-42 PMID: 4113942

Article Info

Journal

Histochemistry

Abbr.

Histochemistry

ISSN

0301-5564

DOI

10.1007/BF00489751

Published

1984-00-00

Pages

465-8

Language

English

Region

Germany

NLM ID

0411300

Subset

External Links

PubMed source DOI: 10.1007/BF00489751

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The Giemsa-11 technique for species-specific chromosome differentiation. A simple stain modification leading to dependable direct and sequential staining procedures.

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