The concentration of the mitochondrial pyruvate carrier in rat liver and heart mitochondria determined with alpha-cyano-beta-(1-phenylindol-3-yl)acrylate.
alpha-Cyano-beta-(1-phenylindol-3-yl)acrylate inhibited pyruvate transport into both liver and heart mitochondria approximately linearly with respect to its concentration until 65% inhibition was achieved. The extent of inhibition was dependent on the mitochondrial protein concentration. By extrapolation of plots of inhibition versus inhibitor concentration to total inhibition, or by mathematical analysis of the plots, the concentration of pyruvate transporter molecules per mg of protein was calculated to be approximately 100 pmol/mg for both heart and liver mitochondria, and the Ki about 7 nM. The data also suggest that pyruvate transport is rate-limiting for pyruvate oxidation by heart mitochondria in State 3, but not by liver mitochondria.
13 references, click to expand
-
Pyruvate and ketone-body transport across the mitochondrial membrane. Exchange properties, pH-dependence and mechanism of the carrier.Biochem J. 1978 Jun 15;172(3):377-87 PMID: 28726
-
Stimulation of pyruvate transport in metabolizing mitochondria through changes in the transmembrane pH gradient induced by glucagon treatment of rats.Biochem J. 1978 Jun 15;172(3):389-98 PMID: 28727
-
Mitochondrial pyruvate transport and its hormonal regulation.Int J Biochem. 1980;11(2):97-105 PMID: 6987111
-
Role of calcium ions in the regulation of intramitochondrial metabolism. Effects of Na+, Mg2+ and ruthenium red on the Ca2+-stimulated oxidation of oxoglutarate and on pyruvate dehydrogenase activity in intact rat heart mitochondria.Biochem J. 1980 Jul 15;190(1):107-17 PMID: 6160850
-
Identification of the protein responsible for pyruvate transport into rat liver and heart mitochondria by specific labelling with [3H]N-phenylmaleimide.Biochem J. 1981 May 15;196(2):471-9 PMID: 7316989
-
Computer stimulation of the effects of alpha-cyano-4-hydroxycinnamate on gluconeogenesis from L-lactate in rat liver cells.Biochem J. 1981 Sep 15;198(3):561-4 PMID: 7326023
-
Transport of pyruvate nad lactate into human erythrocytes. Evidence for the involvement of the chloride carrier and a chloride-independent carrier.Biochem J. 1976 May 15;156(2):193-207 PMID: 942406
-
A re-evaluation of the role of mitochondrial pyruvate transport in the hormonal control of rat liver mitochondrial pyruvate metabolism.Biochem J. 1984 Nov 1;223(3):677-85 PMID: 6095807
-
Aggregation and electrophoretic mobility studies on dissociated cells. II. Effects of ADP and ATP.Exp Cell Res. 1970 Dec;63(2):301-8 PMID: 5490338
-
The mitochondrial pyruvate carrier. Kinetics and specificity for substrates and inhibitors.Biochem J. 1975 Apr;148(1):85-96 PMID: 1156402
-
The specificity and metabolic implications of the inhibition of pyruvate transport in isolated mitochondria and intact tissue preparations by alpha-Cyano-4-hydroxycinnamate and related compounds.Biochem J. 1975 Apr;148(1):97-106 PMID: 1171687
-
The mechanism of the inhibition of the mitochondrial pyruvate transportater by alpha-cyanocinnamate derivatives.Biochem J. 1976 Apr 15;156(1):181-3 PMID: 942399
-
The nature of the changes in liver mitochondrial function induced by glucagon treatment of rats. The effects of intramitochondrial volume, aging and benzyl alcohol.Biochim Biophys Acta. 1982 Sep 15;681(3):429-39 PMID: 7126557
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
