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PMID: 6458333 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Vanadate inhibition of active Ca2+ transport across human red cell membranes.

Biochimica et biophysica acta ·Vol. 648 ·No. 2 ·1981-11-06 ·Pages 145-50

Rossi JP, Garrahan PJ, Rega AF

Abstract

(1) Vanadate (pentavalent vanadium) inhibits with high affinity (K0.5 = 3 microM) the ATP-dependent Ca2+ efflux in reconstituted ghosts from human red cell. (2) To inhibit Ca2+ efflux vanadate has to have access to the inner surface of the cell membrane (3) The inhibitory effect of vanadate is potentiated by intracellular Mg2+ and by intracellular K+. (4) Ca2+ in the external medium antagonizes the inhibitory effect of vanadate.

MeSH Terms

Biological Transport, Active/drug effects Calcium/blood Calcium-Transporting ATPases/blood Erythrocyte Membrane/drug effects,metabolism Erythrocytes/metabolism Humans Kinetics Magnesium/pharmacology Norepinephrine/pharmacology Potassium/pharmacology Vanadates Vanadium/pharmacology

Chemicals

Vanadium Vanadates Calcium-Transporting ATPases Magnesium Potassium Calcium Norepinephrine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rossi J P

Garrahan P J

Rega A F

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0005-2736(81)90029-8

Published

1981-11-06

Pages

145-50

Language

English

Region

Netherlands

NLM ID

0217513

Subset

External Links

PubMed source DOI: 10.1016/0005-2736(81)90029-8

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Vanadate inhibition of active Ca2+ transport across human red cell membranes.

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