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PMID: 6454001 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Correlation between ultrastructural and functional changes in sarcoplasmic reticulum during chronic stimulation of fast muscle.

The Journal of membrane biology ·Vol. 59 ·No. 2 ·1981-04-15 ·Pages 143-9

Heilman C, Müller W, Pette D

Abstract

Chronic indirect stimulation of fast twitch rabbit muscle (tibialis anterior and extensor digitorum longus) with a frequency of 10 Hz induced a progressive transformation of the sarcoplasmic reticulum (SR). Ultrastructural changes as studied by electron microscopy of freeze-fractured vesicles consisted in a decrease of intramembranous particles of the concave (A) face and an increase of particles in the convex (B) face. The asymmetry of the membrane proved to be lowered. Changes in the particle density of the A face were mainly confined to the 7-9 nm particles. Electrophoretic analyses revealed a decrease in the 115,000-Mr Ca2+ transport ATPase. The reduced density of the 7-9 nm particles correlated well with decreased activities in Ca2+-dependent ATPase as well as with decreases in initial and maximum Ca2+ uptake.

MeSH Terms
Animals Calcium-Transporting ATPases/metabolism Electric Stimulation Freeze Fracturing Intracellular Membranes/ultrastructure Male Membrane Proteins/metabolism Molecular Weight Muscle Contraction Muscles/physiology Rabbits Sarcoplasmic Reticulum/physiology,ultrastructure Time Factors
Chemicals
Membrane Proteins Calcium-Transporting ATPases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Heilman C
Müller W
Pette D
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24 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01875712
Published
1981-04-15
Pages
143-9
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01875712 View journal details
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