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PMID: 6453348 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Immune labeling of the D and E regions of human fibrinogen by electron microscopy.

Norton PA, Slayter HS

Abstract

Human fibrinogen was digested with trypsin to yield core fragments D and E, and antibodies were made against the isolated fragments. The Fab' fragments derived from these antibodies were mixed with native fibrinogen, resulting in the formation of soluble immune complexes. These were rotary shadowed with platinum or negatively contrasted with uranyl acetate and examined by electron microscopy. Fab' from anti-D immunoglobulin was found to be attached to the outer nodules of fibrinogen with a frequency of 79% prior to affinity purification and 91% afterward. Fab' from anti-E immunoglobulin attached to the central nodule with a frequency of 78% prior to affinity purification and 82% afterward. The evidence clearly identifies fragment D produced by plasmin or trypsin digestion of fibrinogen with the outer nodules and the single fragment E, with the central nodule.

MeSH Terms
Antibodies Antigen-Antibody Complex Fibrinogen/immunology Fibrinolysin Humans Immunodiffusion Immunoglobulin Fab Fragments Microscopy, Electron Peptide Fragments/immunology Protein Conformation Trypsin
Chemicals
Antibodies Antigen-Antibody Complex Immunoglobulin Fab Fragments Peptide Fragments Fibrinogen Trypsin Fibrinolysin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Norton P A
Slayter H S
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1981-03-00
Pages
1661-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319192
Subset
IM
Grants
PHS HHS · FR-05526 · United States
NIGMS NIH HHS · GM-14237 · United States
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