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PMID: 6452060 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

FUdR induction of the X chromosome fragile site: evidence for the mechanism of folic acid and thymidine inhibition.

American journal of human genetics ·Vol. 33 ·No. 2 ·1981-03-00 ·Pages 234-42

Glover TW

Abstract

Experiments designed to illuminate the mechanism by which folic acid and thymidine inhibit expression of the Xq28 fragile site in human lymphocytes are described. The fragile site is induced by 5-fluorodeoxyuridine (FUdR), a potent inhibitor of thymidylate synthetase, in the presence of otherwise inhibiting concentrations of folic acid but not in the presence of thymidine. These results indicate that the fragile site is expressed because of depletion of deoxythymidine monophosphate (dTMP) available for DNA synthesis.

MeSH Terms
Cells, Cultured DNA/biosynthesis Female Floxuridine/antagonists & inhibitors,pharmacology Folic Acid/pharmacology Humans Intellectual Disability/genetics Lymphocytes/ultrastructure Male Sex Chromosome Aberrations/genetics Sex Chromosomes/drug effects Thymidine/pharmacology Thymidine Monophosphate/metabolism Thymidylate Synthase/antagonists & inhibitors X Chromosome/drug effects
Chemicals
Floxuridine Thymidine Monophosphate DNA Folic Acid Thymidylate Synthase Thymidine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Glover T W
References (13)
13 references, click to expand
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Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1981-03-00
Pages
234-42
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1684940
Subset
IM
Grants
NICHD NIH HHS · HD-07879 · United States
External Links
PubMed source PMC Full Text View journal details
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