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PMID: 6448985 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ATPase activity in rapidly activated skinned muscle fibres.

Pflugers Archiv : European journal of physiology ·Vol. 387 ·No. 2 ·1980-09-00 ·Pages 167-73

Griffiths PJ, Güth K, Kuhn HJ, Rüegg JC

Abstract

The ATPase activity of single mechanically skinned fibres from semitendinosus muscle of Rana esculenta was measured. The method was based on the fluorimetrically detected consumption of NADH inside the fibre. NADH breakdown is caused by the reaction of an ATP regenerating system coupled to an NADH oxidising system. The time resolution was about 5 s. The initial rise in ATPase activity was delayed when a fibre was activated by Ca, and ATPase activity reached its plateau at the same time as fibre tension. An activated fibre that was isolated from the incubation solution by immersing it into oil showed a temporary decrease in tension without corresponding decrease in the ATPase activity.

MeSH Terms
Adenosine Triphosphatases/metabolism Adenosine Triphosphate/biosynthesis Animals Calcium/physiology Calcium-Transporting ATPases/metabolism Enzyme Activation Muscles/enzymology Myosins/physiology Rana esculenta Time Factors
Chemicals
Adenosine Triphosphate Adenosine Triphosphatases Myosins Calcium-Transporting ATPases Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Griffiths P J
Güth K
Kuhn H J
Rüegg J C
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22 references, click to expand
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00584268
Published
1980-09-00
Pages
167-73
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00584268 View journal details
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