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PMID: 6438156 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nonenzymatic glycation of human lens crystallin. Effect of aging and diabetes mellitus.

The Journal of clinical investigation ·Vol. 74 ·No. 5 ·1984-11-00 ·Pages 1742-9

Garlick RL, Mazer JS, Chylack LT, Tung WH, Bunn HF

Abstract

We have examined the nonenzymatic glycation of human lens crystallin, an extremely long-lived protein, from 16 normal human ocular lenses 0.2-99 yr of age, and from 11 diabetic lenses 52-82-yr-old. The glucitol-lysine (Glc-Lys) content of soluble and insoluble crystallin was determined after reduction with H-borohydride followed by acid hydrolysis, boronic acid affinity chromatography, and high pressure cation exchange chromatography. Normal lens crystallin, soluble and insoluble, had 0.028 +/- 0.011 nanomoles Glc-Lys per nanomole crystallin monomer. Soluble and insoluble crystallins had equivalent levels of glycation. The content of Glc-Lys in normal lens crystallin increased with age in a linear fashion. Thus, the nonenzymatic glycation of nondiabetic lens crystallin may be regarded as a biological clock. The diabetic lens crystallin samples (n = 11) had a higher content of Glc-Lys (0.070 +/- 0.034 nmol/nmol monomer). Over an age range comparable to that of the control samples, the diabetic crystallin samples contained about twice as much Glc-Lys. The Glc-Lys content of the diabetic lens crystallin samples did not increase with lens age.

MeSH Terms
Adolescent Adult Aged Aging Crystallins/metabolism Diabetes Mellitus/metabolism Female Glucose/metabolism Humans Infant Lens, Crystalline/metabolism Lysine Male Middle Aged Solubility
Chemicals
Crystallins Glucose Lysine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Garlick R L
Mazer J S
Chylack L T
Tung W H
Bunn H F
References (43)
43 references, click to expand
  1. Lens proteins and aging.
    J Gerontol. 1983 May;38(3):278-86 PMID: 6341444
  2. The principal site of nonenzymatic glycosylation of human serum albumin in vivo.
    J Biol Chem. 1983 May 25;258(10):6142-6 PMID: 6853480
  3. Characterization of glycosylated hemoglobins. Relevance to monitoring of diabetic control and analysis of other proteins.
    J Clin Invest. 1983 May;71(5):1062-72 PMID: 6406542
  4. Nonenzymatic glucosylation and glucose-dependent cross-linking of protein.
    J Biol Chem. 1983 Aug 10;258(15):9406-12 PMID: 6409904
  5. Increased glycosylation of proteins from cataractous lenses in diabetes.
    Diabetologia. 1983 Jul;25(1):36-8 PMID: 6884614
  6. Structural homology of lens crystallins. III. Secondary structure estimation from circular dichroism and prediction from amino acid sequences.
    Biochim Biophys Acta. 1983 Oct 17;748(1):56-67 PMID: 6615851
  7. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  8. [The structure of normal adult human hemoglobins].
    Hoppe Seylers Z Physiol Chem. 1961 Sep 20;325:283-6 PMID: 13872627
  9. A new N-terminal blocking group involving a Schiff base in hemoglobin AIc.
    Biochemistry. 1966 Aug;5(8):2489-503 PMID: 5968556
  10. The optical rotatory dispersion and circular dichroism of calf lens alpha-crystallin.
    J Biol Chem. 1967 Jul 10;242(13):3234-6 PMID: 6027797
  11. Structure of hemoglobin AIc: nature of the N-terminal beta chain blocking group.
    Biochem Biophys Res Commun. 1968 Jul 11;32(1):86-93 PMID: 4874776
  12. The amino acid sequence of -crystallin (fraction II) from calf lens.
    Biochem J. 1972 Jul;128(4):961-70 PMID: 4674126
  13. Micro method for determination of borohydride with NAD+.
    Anal Biochem. 1973 Aug;54(2):554-60 PMID: 4353368
  14. The distribution of the soluble proteins in the calf lens.
    Exp Eye Res. 1973 Dec 10;17(5):417-26 PMID: 4783179
  15. Physical and chemical changes in alpha-crystallin during maturation of lens fibers.
    Exp Eye Res. 1974 Apr;18(4):383-93 PMID: 4834045
  16. Circular dichroism and optical rotatory dispersion of the aggregates of purified polypeptides of alpha-crystallin.
    Exp Eye Res. 1974 Jul;19(1):49-57 PMID: 4412450
  17. The microscopic protein structure of the lens with a theory for cataract formation as determined by Raman spectroscopy of intact bovine lenses.
    Invest Ophthalmol. 1975 May;14(5):380-96 PMID: 1126827
  18. Further identification of the nature and linkage of the carbohydrate in hemoglobin A1c.
    Biochem Biophys Res Commun. 1975 Nov 3;67(1):103-9 PMID: 1201013
  19. Structural proteins of the mammalian lens: a review with emphasis on changes in development, aging and cataract.
    Exp Eye Res. 1976 Jan;22(1):1-73 PMID: 767125
  20. The amino acid sequence of the A chain of human alpha-crystallin.
    FEBS Lett. 1975 Oct 15;58(1):310-3 PMID: 817940
  21. The biosynthesis of human hemoglobin A1c. Slow glycosylation of hemoglobin in vivo.
    J Clin Invest. 1976 Jun;57(6):1652-9 PMID: 932199
  22. Correlation of glucose regulation and hemoglobin AIc in diabetes mellitus.
    N Engl J Med. 1976 Aug 19;295(8):417-20 PMID: 934240
  23. In vitro synthesis of hemoglobin AIc.
    FEBS Lett. 1976 Dec 1;71(2):356-60 PMID: 12012
  24. The primary structure of the B2 chain of human alpha-crystallin.
    FEBS Lett. 1977 Feb 15;74(1):82-4 PMID: 838078
  25. Structure of carbohydrate of hemoglobin AIc.
    J Biol Chem. 1977 May 10;252(9):2992-7 PMID: 853040
  26. Glycosylated hemoglobins and long-term blood glucose control in diabetes mellitus.
    J Clin Endocrinol Metab. 1977 May;44(5):859-64 PMID: 858776
  27. The vertebrate eye lens.
    Science. 1977 Jul 8;197(4299):127-38 PMID: 877544
  28. Conformation of gamma-crystallins of the calf lens: effects of temperature and denaturing agents.
    Exp Eye Res. 1977 Aug;25(2):199-208 PMID: 913511
  29. The glycosylation of hemoglobin: relevance to diabetes mellitus.
    Science. 1978 Apr 7;200(4337):21-7 PMID: 635569
  30. Diabetic cataract formation: potential role of glycosylation of lens crystallins.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2918-22 PMID: 275862
  31. Isolation and physical characterization of bovine lens crystallins.
    Int J Pept Protein Res. 1979 Apr;13(4):409-17 PMID: 457334
  32. Glucosylation of human lens protein and cataractogenesis.
    Biochem Biophys Res Commun. 1979 Aug 28;89(4):1260-6 PMID: 496956
  33. Enhanced nonenzymatic glucosylation of human serum albumin in diabetes mellitus.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4258-61 PMID: 291961
  34. Sites of nonenzymatic glycosylation of human hemoglobin A.
    J Biol Chem. 1980 Apr 10;255(7):3120-7 PMID: 7358733
  35. Role of glycosylation in protein disulfide formation and cataractogenesis.
    Exp Eye Res. 1980 Jul;31(1):9-19 PMID: 7428839
  36. Measurement of glycosylated amino acids and peptides from urine of diabetic patients using affinity chromatography.
    Diabetes. 1980 Dec;29(12):1044-7 PMID: 6777222
  37. Nonenzymatic browning in vivo: possible process for aging of long-lived proteins.
    Science. 1981 Jan 30;211(4481):491-3 PMID: 6779377
  38. Nonenzymatic glycosylation of bovine lens crystallins. Effect of aging.
    J Biol Chem. 1981 May 25;256(10):5176-80 PMID: 7228874
  39. Effects of age and diabetes mellitus on the solubility and nonenzymatic glucosylation of human skin collagen.
    J Clin Invest. 1981 Jun;67(6):1630-5 PMID: 6787079
  40. Bovine heart mitochondrial transhydrogenase catalyzes an exchange reaction between NADH and NAD+.
    J Biol Chem. 1981 Jul 25;256(14):7401-8 PMID: 7251601
  41. Primary structure of the bovine beta-crystallin Bp chain. Internal duplication and homology with gamma-crystallin.
    Eur J Biochem. 1981 Dec;121(1):83-91 PMID: 7035168
  42. Spectroscopic investigations of bovine lens crystallins. 1. Circular dichroism and intrinsic fluorescence.
    Biochemistry. 1982 Apr 13;21(8):1847-52 PMID: 7082650
  43. Non-enzymatic glycosylation and browning of proteins in diabetes.
    Clin Endocrinol Metab. 1982 Jul;11(2):431-52 PMID: 6754164
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI111592
Published
1984-11-00
Pages
1742-9
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC425353
Subset
IM
Grants
NIADDK NIH HHS · AM-18223 · United States
External Links
PubMed source DOI: 10.1172/JCI111592 PMC Full Text View journal details
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