Susceptibility of Mycobacterium tuberculosis to pyrazinamide and its relationship to pyrazinamidase activity.

Antimicrobial agents and chemotherapy ·Vol. 24 ·No. 4 ·1983-10-00 ·Pages 600-1

Butler WR, Kilburn JO

Abstract

Pyrazinamidase activity has been associated with pyrazinamide-susceptible Mycobacterium tuberculosis strains. The detection of pyrazinamidase activity by the Wayne method was found to be of limited value when compared with the results of standard pyrazinamide susceptibility tests, especially when a high level of pyrazinamide resistance was found. When resistance to pyrazinamide reached a level of 150 to 200 micrograms/ml, there was too much variability in Wayne test results to accurately define pyrazinamide susceptibility.

MeSH Terms

Amidohydrolases/metabolism Drug Resistance, Microbial Mutation Mycobacterium tuberculosis/drug effects,enzymology Pyrazinamide/pharmacology

Chemicals

Pyrazinamide Amidohydrolases pyrazinamide deamidase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Butler W R

Kilburn J O

References (8)

8 references, click to expand

Pyrazinamide susceptibility and amidase activity of tubercle bacilli.
Am Rev Respir Dis. 1967 Mar;95(3):461-9 PMID: 4225184
Determination of drug susceptibility of mycobacteria to pyrazinamide in 7H10 agar.
Am Rev Respir Dis. 1967 Nov;96(5):1072-5 PMID: 4964643
A simple way of detecting pyrazinamide resistance.
Tubercle. 1972 Jun;53(2):128-31 PMID: 4261137
Simple pyrazinamidase and urease tests for routine identification of mycobacteria.
Am Rev Respir Dis. 1974 Jan;109(1):147-51 PMID: 4203284
[Sensitivity of tubercle bacilli to pyrazinamide determined on the basis of their sensitivity to nicotinamide and their pyrazinamidase and nictinamidase activities].
Gruzlica. 1974 Aug;42(8):773-7 PMID: 4277272
Use of pyrazinamidase activity on Mycobacterium tuberculosis as a rapid method for determination of pyrazinamide susceptibility.
Antimicrob Agents Chemother. 1981 Oct;20(4):556-7 PMID: 6805419
Improved method for testing susceptibility of Mycobacterium tuberculosis to pyrazinamide.
J Clin Microbiol. 1982 Dec;16(6):1106-9 PMID: 6819309
Activation of pyrazinamide and nicotinamide in acidic environments in vitro.
Am Rev Tuberc. 1954 Oct;70(4):748-54 PMID: 13197751

Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.24.4.600

Published

1983-10-00

Pages

600-1

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC185381

Subset

External Links

PubMed source DOI: 10.1128/AAC.24.4.600 PMC Full Text View journal details

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Susceptibility of Mycobacterium tuberculosis to pyrazinamide and its relationship to pyrazinamidase activity.

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