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PMID: 6417660 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Electrophoretic analysis of human HLA-DR antigens from HLA-DR4 homozygous cell lines: correlation between beta-chain diversity and HLA-D.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 22 ·1983-11-00 ·Pages 6962-6

Nepom BS, Nepom GT, Mickelson E, Antonelli P, Hansen JA

Abstract

Two-dimensional gel electrophoresis of immunoprecipitated human HLA-DR antigens from cells expressing the HLA-DR4 haplotype shows distinct clustering of beta-chain patterns. Six unique electrophoretic variants were observed among 17 HLA-DR4 homozygous cell lines (HCL) analyzed. These patterns correlate precisely with the HLA-D phenotype of the HCL donor as determined by reactivity in mixed lymphocyte culture. All DR4 HCL that belong to one of the well-defined HLA-D antigen groups (Dw4, Dw10, LD "40", LD "DYT", LD "KT2", or LD "TAS") have identical DR beta-chain patterns; DR4 HCL belonging to different HLA-D antigen groups do not. The concordance of the functional expression in mixed lymphocyte culture of a specific D phenotype with a distinct DR beta-chain pattern on gel analysis provides a direct structural basis for understanding the genetic control of HLA-D polymorphisms; HLA-D specificities as revealed by T-cell recognition in mixed lymphocyte culture thus might be accounted for by DR beta-chain polymorphisms. The extent of this beta-chain diversity within a single DR haplotype may aid in understanding variations in Ia-regulated functions, such as Ir gene control and certain disease susceptibilities.

MeSH Terms
B-Lymphocytes/immunology Cell Line Electrophoresis, Polyacrylamide Gel Genes, MHC Class II HLA-DR Antigens HLA-DR4 Antigen Histocompatibility Antigens Class II/isolation & purification Homozygote Humans Macromolecular Substances
Chemicals
HLA-DR Antigens HLA-DR4 Antigen Histocompatibility Antigens Class II Macromolecular Substances
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nepom B S
Nepom G T
Mickelson E
Antonelli P
Hansen J A
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.22.6962
Published
1983-11-00
Pages
6962-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390106
Subset
IM
Grants
NCI NIH HHS · CA 18029 · United States
NHLBI NIH HHS · HL 17265 · United States
NCRR NIH HHS · RR 05761 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.22.6962 PMC Full Text View journal details
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