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PMID: 6413489 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Involvement of lactose enzyme II of the phosphotransferase system in rapid expulsion of free galactosides from Streptococcus pyogenes.

Journal of bacteriology ·Vol. 156 ·No. 1 ·1983-10-00 ·Pages 236-42

Reizer J, Saier MH

Abstract

Streptococcus pyogenes accumulated thiomethyl-beta-galactoside as the 6-phosphate ester due to the action of the phosphoenolpyruvate:lactose phosphotransferase system. Subsequent addition of glucose resulted in rapid efflux of the free galactoside after intracellular dephosphorylation (inducer expulsion). Efflux was shown to occur in the apparent absence of the galactose permease, but was inhibited by substrate analogs of the lactose enzyme II and could not be demonstrated in a mutant of S. lactis ML3 which lacked this enzyme. The results suggest that the enzymes II of the phosphotransferase system can catalyze the rapid efflux of free sugar under appropriate physiological conditions.

MeSH Terms
Biological Transport/drug effects Galactosides/metabolism Glucose/metabolism Lactococcus lactis/enzymology Membrane Transport Proteins/metabolism Methylgalactosides/metabolism Methylglycosides/metabolism Mutation Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism Streptococcus pyogenes/enzymology Thiogalactosides/metabolism Thioglycosides/metabolism
Chemicals
Galactosides Membrane Transport Proteins Methylgalactosides Methylglycosides Thiogalactosides Thioglycosides thiomethylgalactoside Phosphoenolpyruvate Sugar Phosphotransferase System phosphoenolpyruvate-lactose dependent phosphotransferase system Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reizer J
Saier M H
References (31)
31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.156.1.236-242.1983
Published
1983-10-00
Pages
236-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215075
Subset
IM
Grants
NIAID NIH HHS · 1 ROI AI 14176-03 · United States
External Links
PubMed source DOI: 10.1128/jb.156.1.236-242.1983 PMC Full Text View journal details
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