Home LiteratureArticle Details
PMID: 6401692 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evaluation of Pseudomonas aeruginosa exotoxin A and elastase as virulence factors in acute lung infection.

Infection and immunity ·Vol. 39 ·No. 1 ·1983-01-00 ·Pages 198-201

Blackwood LL, Stone RM, Iglewski BH, Pennington JE

Abstract

Acute Pseudomonas aeruginosa pneumonia was established in guinea pigs by intratracheal instillation of bacteria. Challenge strains included PAO-1, a strain known to produce exotoxin A, alkaline protease, and elastase, and several PAO-1 mutants deficient in either biologically active exotoxin A or elastase production. Survival, intrapulmonary killing of bacteria, and blood cultures were compared among the groups. Strains of P. aeruginosa deficient in active elastase production appeared to be less virulent than the parent strain and were more easily cleared from the lung. Opposite results were obtained for the exotoxin A-deficient mutants. These data suggest that elastase, but not exotoxin A, was an important virulence factor during acute pneumonia due to P. aeruginosa.

MeSH Terms
ADP Ribose Transferases Acute Disease Animals Bacterial Toxins Exotoxins/toxicity Guinea Pigs Pancreatic Elastase/toxicity Pneumonia/etiology Pseudomonas aeruginosa/enzymology,pathogenicity Virulence Virulence Factors
Chemicals
Bacterial Toxins Exotoxins Virulence Factors ADP Ribose Transferases toxA protein, Pseudomonas aeruginosa Pancreatic Elastase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Blackwood L L
Stone R M
Iglewski B H
Pennington J E
References (21)
21 references, click to expand
  1. Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase.
    J Bacteriol. 1980 Jun;142(3):836-42 PMID: 6769912
  2. Microscopic characterization of rabbit lung damage produced by Pseudomonas aeruginosa proteases.
    Infect Immun. 1979 Jan;23(1):150-9 PMID: 106001
  3. Effectiveness of immunization with single and multi-component vaccines prepared from a common antigen (OEP), protease and elastase toxoids of Pseudomonas aeruginosa on protection against hemorrhagic pneumonia in mink due to P. aeruginosa.
    Jpn J Exp Med. 1978 Apr;48(2):111-33 PMID: 101698
  4. Pseudomonas aeruginosa exotoxin A.
    N Engl J Med. 1980 Jun 12;302(24):1360-2 PMID: 6769039
  5. Effect of iron on yields of exotoxin A in cultures of Pseudomonas aeruginosa PA-103.
    Infect Immun. 1978 Mar;19(3):785-91 PMID: 417030
  6. Lipopolysaccharide pseudomonas vaccine: efficacy against pulmonary infection with Pseudomonas aeruginosa.
    J Infect Dis. 1979 Jul;140(1):73-80 PMID: 110888
  7. Contribution of toxin A and elastase to virulence of Pseudomonas aeruginosa in chronic lung infections of rats.
    Infect Immun. 1982 Jun;36(3):1223-8 PMID: 6807846
  8. In Vivo Studies with the Partially Purified Protease (Elastase) from Pseudomonas aeruginosa.
    Infect Immun. 1970 Nov;2(5):583-9 PMID: 16557881
  9. Assessment of protease (elastase) as a Pseudomonas aeruginosa virulence factor in experimental mouse burn infection.
    Infect Immun. 1979 Apr;24(1):181-7 PMID: 110690
  10. Isolation and characterization of a Pseudomonas aeruginosa mutant producing a nontoxic, immunologically crossreactive toxin A protein.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7199-203 PMID: 6261247
  11. Evaluation of a new polyvalent Pseudomonas vaccine in respiratory infections.
    Infect Immun. 1979 Sep;25(3):1029-34 PMID: 115786
  12. NAD-dependent inhibition of protein synthesis by Pseudomonas aeruginosa toxin,.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2284-8 PMID: 166383
  13. Pseudomonas aeruginosa infections: persisting problems and current research to find new therapies.
    Ann Intern Med. 1975 Jun;82(6):819-31 PMID: 806246
  14. Production of protease and elastase by Pseudomonas aeruginosa strains isolated from patients.
    Infect Immun. 1977 Mar;15(3):679-85 PMID: 15945
  15. Corneal infections in mice with toxin A and elastase mutants of Pseudomonas aeruginosa.
    J Infect Dis. 1980 Oct;142(4):547-55 PMID: 6904461
  16. Chromosomal genetics of Pseudomonas.
    Microbiol Rev. 1979 Mar;43(1):73-102 PMID: 111024
  17. Extracellular toxins of Pseudomonas aeruginosa.
    J Infect Dis. 1974 Nov;130 Suppl(0):S94-9 PMID: 4370620
  18. Influence of mucoid coating on clearance of Pseudomonas aeruginosa from lungs.
    Infect Immun. 1981 May;32(2):443-8 PMID: 6788696
  19. Role of exotoxin and protease as possible virulence factors in experimental infections with Pseudomonas aeruginosa.
    Infect Immun. 1978 Mar;19(3):839-45 PMID: 417031
  20. Elastin structure, biosynthesis, and relation to disease states.
    N Engl J Med. 1981 Mar 5;304(10):566-79 PMID: 7005671
  21. The role of exotoxins in the pathogenesis of Pseudomonas aeruginosa infections.
    J Infect Dis. 1980 Oct;142(4):626-30 PMID: 6777439
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.39.1.198-201.1983
Published
1983-01-00
Pages
198-201
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC347925
Subset
IM
Grants
NIAID NIH HHS · AI-14671 · United States
NIAID NIH HHS · AI-15411 · United States
External Links
PubMed source DOI: 10.1128/iai.39.1.198-201.1983 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2