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PMID: 6396164 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence and analysis of deletion mutants of the Escherichia coli gpt gene in plasmid pSV2 gpt.

Gene ·Vol. 31 ·No. 1-3 ·1984-11-00 ·Pages 309-13

Jagadeeswaran P, Ashman CR, Roberts S, Langenberg J

Abstract

The complete sequence of the HindIII-BamHI/BglII fragment of plasmid pSV2 gpt, which contains the E. coli gpt gene, has been determined and used to predict the amino acid sequence of the protein. The nucleotide sequence was used to create a map of eight gpt gene deletion mutants which were generated in mammalian cells.

MeSH Terms

Animals Bacterial Proteins/genetics Base Sequence Chromosome Deletion Chromosomes, Bacterial/ultrastructure Cricetinae Cricetulus Escherichia coli/enzymology,genetics Genes Genes, Bacterial Pentosyltransferases/genetics Plasmids

Chemicals

Bacterial Proteins Pentosyltransferases xanthine phosphoribosyltransferase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Jagadeeswaran P

Ashman C R

Roberts S

Langenberg J

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/0378-1119(84)90228-2

Published

1984-11-00

Pages

309-13

Language

English

Region

Netherlands

NLM ID

7706761

Subset

Grants

NCI NIH HHS · CA 31781 · United States

Databases

GENBANK

M12907

External Links

PubMed source DOI: 10.1016/0378-1119(84)90228-2 View journal details

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Nucleotide sequence and analysis of deletion mutants of the Escherichia coli gpt gene in plasmid pSV2 gpt.

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