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PMID: 6389446 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The ubiquitous localization of type I hexokinase in rat peripheral nerves, smooth muscle cells and epithelial cells.

The Histochemical journal ·Vol. 16 ·No. 10 ·1984-10-00 ·Pages 1113-23

Lawrence GM, Walker DG, Trayer IP

Abstract

An indirect immunoperoxidase technique has been used to determine the localization of type I hexokinase in a wide variety of Carnoy-fixed, paraffin-embedded rat tissues. The results suggest that the widespread tissue distribution of the isoenzyme is due to its ubiquitous localization in the nervous, smooth muscle and epithelial components of each tissue. The majority of the immunostaining was confined to cells with substantial energy requirements which are probably mainly satisfied through the breakdown of glucose. This observation is consistent with the known predominance of type I hexokinase in the central nervous system and with the regulatory role allotted to it in this tissue.

MeSH Terms
Animals Epithelium/enzymology Female Hexokinase/metabolism Immunoenzyme Techniques Isoenzymes/metabolism Male Muscle, Smooth/enzymology Peripheral Nerves/enzymology Rats Rats, Inbred Strains Tissue Distribution
Chemicals
Isoenzymes Hexokinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lawrence G M
Walker D G
Trayer I P
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21 references, click to expand
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Article Info
Journal
The Histochemical journal
Abbr.
Histochem J
ISSN
0018-2214
DOI
10.1007/BF01002898
Published
1984-10-00
Pages
1113-23
Language
English
Region
Netherlands
NLM ID
0163161
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01002898
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