Home LiteratureArticle Details
PMID: 6383355 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mode of action of a bactericidal protein induced in the haemolymph of Sarcophaga peregrina (flesh-fly) larvae.

The Biochemical journal ·Vol. 222 ·No. 1 ·1984-08-15 ·Pages 119-24

Okada M, Natori S

Abstract

The mode of action of a bactericidal protein (sarcotoxin I) purified from the haemolymph of Sarcophaga peregrina (flesh-fly) larvae was studied, focusing attention on its effect on the function of the membrane of Escherichia coli. Sarcotoxin I almost completely blocked the uptakes of tetraphenylphosphonium ion and proline, which are known to be driven by a membrane potential, indicating that this protein disrupts the membrane potential of bacteria.

MeSH Terms
Animals Cell Membrane/drug effects Diptera/analysis Escherichia coli/drug effects,metabolism,ultrastructure Hemolymph/analysis Insect Hormones/blood,pharmacology Insect Proteins Larva/analysis Microscopy, Electron, Scanning Onium Compounds/metabolism Organophosphorus Compounds/metabolism Proline/metabolism Sodium Dodecyl Sulfate/pharmacology
Chemicals
Insect Hormones Insect Proteins Onium Compounds Organophosphorus Compounds Sodium Dodecyl Sulfate sarcotoxin I protein, Sarcophaga peregrina Proline tetraphenylphosphonium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Okada M
Natori S
References (15)
15 references, click to expand
  1. Mitomycin C-sensitive mutant of Eschericha coli K-12.
    J Bacteriol. 1968 Mar;95(3):1191-2 PMID: 4868359
  2. Mutants of Escherichia coli requiring methionine or vitamin B12.
    J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
  3. Transport of sugars and amino acids in bacteria. VI. Changes induced by valine in the branched chain amino acid transport systems of Escherichia coli.
    J Biochem. 1973 Jun;73(6):1149-61 PMID: 4579540
  4. [Demonstration by lauryl-sulfate of some early modifications of E. coli membrane induced by T4 phages, ghosts of T4 phages and colicin K].
    Biochimie. 1974;56(2):221-30 PMID: 4602284
  5. Phase transitions of phospholipid bilayers from an unsaturated fatty acid auxotroph of Escherichia coli.
    Biochim Biophys Acta. 1977 May 2;466(3):393-401 PMID: 322716
  6. Proline transport carrier-defective mutants of Escherichia coli K-12: properties and mapping.
    J Bacteriol. 1978 Oct;136(1):5-9 PMID: 361707
  7. The measurement of membrane potential and deltapH in cells, organelles, and vesicles.
    Methods Enzymol. 1979;55:547-69 PMID: 37402
  8. Sequence and specificity of two antibacterial proteins involved in insect immunity.
    Nature. 1981 Jul 16;292(5820):246-8 PMID: 7019715
  9. Use of lipophilic cation-permeable mutants for measurement of transmembrane electrical potential in metabolizing cells of Escherichia coli.
    J Bacteriol. 1981 Nov;148(2):399-405 PMID: 6795176
  10. Insect immunity. Purification and properties of three inducible bactericidal proteins from hemolymph of immunized pupae of Hyalophora cecropia.
    Eur J Biochem. 1980 May;106(1):7-16 PMID: 7341234
  11. Effects of hemolymph from immune and non-immune larvae of Galleria mellonella on the ultra-structure of Pseudomonas aeruginosa.
    Dev Comp Immunol. 1982 Summer;6(3):433-40 PMID: 6813153
  12. Insect immunity: isolation and structure of cecropin D and four minor antibacterial components from Cecropia pupae.
    Eur J Biochem. 1982 Sep;127(1):207-17 PMID: 7140755
  13. Insect immunity: isolation and structure of cecropins B and D from pupae of the Chinese oak silk moth, Antheraea pernyi.
    Eur J Biochem. 1982 Sep;127(1):219-24 PMID: 6754375
  14. Purification and characterization of an antibacterial protein from haemolymph of Sarcophaga peregrina (flesh-fly) larvae.
    Biochem J. 1983 Jun 1;211(3):727-34 PMID: 6882366
  15. Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1514-8 PMID: 4268097
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2220119
Published
1984-08-15
Pages
119-24
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144151
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2220119 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2