Home LiteratureArticle Details

PMID: 6380609 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Review

Microinjection of cultured cells using red-cell-mediated fusion and osmotic lysis of pinosomes: a review of methods and applications.

Bioscience reports ·Vol. 4 ·No. 6 ·1984-06-00 ·Pages 451-66

McElligott MA, Dice JF

Abstract

Proteins and other macromolecules can be injected into cultured cells by several different methods. Here we review the strengths and limitations of two of these methods, red-cell-mediated microinjection and osmotic lysis of pinosomes, and indicate how they may be successfully applied to the study of cultured cells.

MeSH Terms

Animals Cells, Cultured Endocytosis Erythrocyte Membrane Membrane Fusion/drug effects Microinjections/methods Osmolar Concentration Polyethylene Glycols/pharmacology Proteins/administration & dosage

Chemicals

Proteins Polyethylene Glycols

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

McElligott M A

Dice J F

Article Info

Journal

Bioscience reports

Abbr.

Biosci Rep

ISSN

0144-8463

DOI

10.1007/BF01122221

Published

1984-06-00

Pages

451-66

Language

English

Region

England

NLM ID

8102797

Subset

Grants

NIA NIH HHS · AG02783 · United States

External Links

PubMed source DOI: 10.1007/BF01122221 View journal details

Analysis Services

Microinjection of cultured cells using red-cell-mediated fusion and osmotic lysis of pinosomes: a review of methods and applications.

Contact

WeChat Official Account