Binding and location of AMP deaminase in rabbit psoas muscle myofibrils.

It is shown that an interaction exists between AMP deaminase (EC 3.5.4.6) and myofibrils that is sufficiently strong (Kd congruent to 10(-10) M) for more than 99% of the binding sites for the enzyme to be filled in vivo. The binding is not strong enough, however, to stop removal of the enzyme during the extensive washing normally used in the preparation of myofibrils. Fluorescent antibodies to the enzyme label myofibrils close to the junction of the A- and I-bands. The invariance of the position of the antibody stripes at this site, over a range of sarcomere lengths, indicates that the enzyme is attached to the A-band. The intensity of the fluorescence declines in parallel with dissociation of the enzyme. In this muscle, the number of AMP deaminase binding sites per thick filament is approximately six, suggesting that the enzyme is located at a single axial position in each half A-band. Electron microscopy of negatively stained, antibody-labelled myofibrils reveals the distance between the AMP deaminase sites at opposite ends of an A-band to be 1.69(+/- 0.02 micron). Since the length of the A-band is 1.57 micron, the binding site for the enzyme must be significantly beyond where thick filaments have previously been thought to end.