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PMID: 6370962 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Activation of trehalase by membrane-depolarizing agents in yeast vegetative cells and ascospores.

Journal of bacteriology ·Vol. 158 ·No. 1 ·1984-04-00 ·Pages 337-9

Thevelein JM

Abstract

The membrane-depolarizing agents 2,4-dinitrophenol, carbonylcyanide m-chlorophenylhydrazone, and nystatin are known to cause a rapid increase in the cyclic AMP level in fungal cells. Addition of these proton ionophores to yeast stationary-phase cells or ascospores causes an immediate 10-fold increase in trehalase activity. This observation is in agreement with a role for cyclic AMP-induced phosphorylation in the activation process of trehalase. It also provides an explanation for previous results on the induction of trehalose breakdown by 2,4-dinitrophenol in resting yeast cells.

MeSH Terms
2,4-Dinitrophenol Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Dinitrophenols/pharmacology Enzyme Activation/drug effects Fungi/enzymology Glucose/pharmacology Nitriles/pharmacology Nystatin/pharmacology Phycomyces/enzymology Saccharomyces cerevisiae/enzymology Sodium Cyanide/pharmacology Spores, Fungal/enzymology Trehalase/metabolism
Chemicals
Dinitrophenols Nitriles Nystatin Carbonyl Cyanide m-Chlorophenyl Hydrazone Trehalase Glucose Sodium Cyanide 2,4-Dinitrophenol
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Thevelein J M
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20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.1.337-339.1984
Published
1984-04-00
Pages
337-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215419
Subset
IM
Grants
NIADDK NIH HHS · AM 27121-03 · United States
External Links
PubMed source DOI: 10.1128/jb.158.1.337-339.1984 PMC Full Text View journal details
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