DNA replication regulated by the priming promoter.

Nucleic acids research ·Vol. 12 ·No. 6 ·1984-03-26 ·Pages 2641-8

Panayotatos N

Abstract

ColE1 derivative plasmids were constructed in which the natural promoter that primes replication or, in addition, the region coding for the RNA I control element had been deleted. In all of these molecules priming of the origin was effected by read-through transcription from constitutive or inducible (lacUV5) promoters inserted farther upstream. In the latter case, regulation of lac repressor activity with IPTG resulted in controlled plasmid levels in vivo. These results indicated that, at least in the absence of other control elements, regulation of the priming promoter was sufficient to control DNA replication and determine plasmid copy number.

MeSH Terms

Base Sequence Chromosome Deletion DNA Replication Escherichia coli/genetics Operon Plasmids Repressor Proteins/genetics Transcription, Genetic

Chemicals

Repressor Proteins

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Panayotatos N

References (14)

14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.6.2641

Published

1984-03-26

Pages

2641-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318696

Subset

External Links

PubMed source DOI: 10.1093/nar/12.6.2641 PMC Full Text View journal details

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DNA replication regulated by the priming promoter.

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