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PMID: 6369246 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The DNA sequence of argI from Escherichia coli K12.

Nucleic acids research ·Vol. 11 ·No. 23 ·1983-12-10 ·Pages 8509-18

Bencini DA, Houghton JE, Hoover TA, Foltermann KF, Wild JR, O'Donovan GA

Abstract

The argI gene from E. coli K12 has been sequenced. It contains an open reading frame of 1002 bases which encodes a polypeptide of 334 amino acids. Three such polypeptides are required to form the functional catalytic trimer (c3) of ornithine transcarbamoylase (OTCase-1, EC 2.1.3.3). The molecular mass of the mature trimer deduced from the amino acid sequence is 114,465 daltons. An altered form of argI was produced when a 1.6 kilobase DdeI fragment was subcloned into the HincII site of plasmid pUC8 extending the open reading frame an additional 20 nucleotides. It has been previously reported that the amino-terminal region of the respective polypeptides of argI, argF, and pyrB of E. coli possessed significant homology. In contrast, the homologous promoter/operator regions of argI and argF did not appear to share any homologies with pyrB. However, a closer scrutiny of the nucleotide sequence immediately preceding the pyrBI attenuator revealed a remarkable similarity to the argI and argF control region.

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Bacterial/genetics Escherichia coli/enzymology,genetics Genes Genes, Bacterial Ornithine Carbamoyltransferase/genetics Plasmids

Chemicals

DNA, Bacterial Ornithine Carbamoyltransferase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Bencini D A

Houghton J E

Hoover T A

Foltermann K F

Wild J R

O'Donovan G A

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24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.23.8509

Published

1983-12-10

Pages

8509-18

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326599

Subset

Grants

NIGMS NIH HHS · 1.R01 GM 29152-01 · United States

Databases

GENBANK

X00210

External Links

PubMed source DOI: 10.1093/nar/11.23.8509 PMC Full Text View journal details

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