Genetic mapping of Ty elements in Saccharomyces cerevisiae.
We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a reciprocal exchange using homology at the LEU2 locus or at the various Ty elements that are dispersed throughout the S. cerevisiae genome. These transformants were used to map genetically sites of several Ty elements. The 24 transformants recovered at Ty sites define 19 distinct loci. Seven of these were placed on the genetic map. Two classes of Ty elements were identified in these experiments: a Ty1-17 class and Ty elements different from Ty1-17. Statistical analysis of the number of transformants at each class of Ty elements shows that there is preferential integration of the CV9 plasmid into the Ty1-17 class.
33 references, click to expand
-
Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.Biochemistry. 1970 Oct 27;9(22):4428-40 PMID: 4919547
-
Transposable elements in yeast.Int Rev Cytol. 1983;83:1-25 PMID: 6315625
-
Detection of specific sequences among DNA fragments separated by gel electrophoresis.J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
-
The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA.Methods Cell Biol. 1975;12:335-51 PMID: 1105070
-
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.Gene. 1977;2(2):95-113 PMID: 344137
-
Transformation of yeast.Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
-
Evidence for transposition of dispersed repetitive DNA families in yeast.Cell. 1979 Apr;16(4):739-51 PMID: 378399
-
Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila.Cell. 1979 Jun;17(2):415-27 PMID: 110463
-
Polymorphisms in the chromosomal locations of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila.Cell. 1979 Jun;17(2):429-39 PMID: 110464
-
Properties of yeast transformation.Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1305-13 PMID: 385230
-
Middle repetitive DNA: a fluid component of the Drosophila genome.Proc Natl Acad Sci U S A. 1979 Dec;76(12):6274-8 PMID: 118464
-
Recovery of DNA segments from agarose gels.Anal Biochem. 1980 Jan 15;101(2):339-41 PMID: 6244756
-
Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication.Nature. 1980 Jul 24;286(5771):352-6 PMID: 6250062
-
The yeast transposon Ty1 generates duplications of target DNA on insertion.Nature. 1980 Jul 24;286(5771):414-8 PMID: 6250065
-
DNA rearrangements associated with a transposable element in yeast.Cell. 1980 Aug;21(1):239-49 PMID: 6250713
-
The origins of gene instability in yeast.Science. 1980 Sep 19;209(4463):1375-80 PMID: 6251544
-
Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes.Cell. 1980 Nov;22(2 Pt 2):427-36 PMID: 6256080
-
Intrachromosomal gene conversion in yeast.Nature. 1981 Jan 15;289(5794):144-8 PMID: 7005693
-
A family of long reiterated DNA sequences, one copy of which is next to the human beta globin gene.Nucleic Acids Res. 1980 Dec 20;8(24):6113-28 PMID: 6258162
-
Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II.Cell. 1981 Feb;23(2):605-14 PMID: 6258806
-
Transposable elements (Ty) in yeast.Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:575-80 PMID: 6266751
-
Characterization of the yeast mobile element Ty1.Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:609-17 PMID: 6266754
-
Sequence variation in dispersed repetitive sequences in Saccharomyces cerevisiae.J Mol Biol. 1981 Feb 5;145(4):619-32 PMID: 7021854
-
Sequence variation in the LEU2 region of the saccharomyces cerevisiae genome.Gene. 1981 Dec;16(1-3):133-9 PMID: 6282687
-
Genomic representation of the Hind II 1.9 kb repeated DNA.Nucleic Acids Res. 1982 May 25;10(10):3221-39 PMID: 6285293
-
Genetic properties of chromosomally integrated 2 mu plasmid DNA in yeast.Cell. 1982 Jun;29(2):573-84 PMID: 6288264
-
Differing levels of dispersed repetitive DNA among closely related species of Drosophila.Proc Natl Acad Sci U S A. 1982 Aug;79(15):4570-4 PMID: 6956880
-
Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae.Mol Cell Biol. 1982 Sep;2(9):1080-7 PMID: 6757721
-
Rearranged mitochondrial genes in the yeast nuclear genome.Nature. 1983 Jan 27;301(5898):296-301 PMID: 6296698
-
Nucleotide sequence of yeast LEU2 shows 5'-noncoding region has sequences cognate to leucine.Cell. 1982 Dec;31(2 Pt 1):319-25 PMID: 6297759
-
Chromosomes XIV and XVII of Saccharomyces cerevisiae constitute a single linkage group.Mol Cell Biol. 1982 Nov;2(11):1399-409 PMID: 6761582
-
Recombination of plasmids into the Saccharomyces cerevisiae chromosome is reduced by small amounts of sequence heterogeneity.Mol Cell Biol. 1983 Jul;3(7):1204-11 PMID: 6350848
-
Repetitive sequences in isolated Thomas circles from Drosophila melanogaster.Cold Spring Harb Symp Quant Biol. 1974;38:371-81 PMID: 4208788
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