Genetic evidence for glucitol-specific enzyme III, an essential phosphocarrier protein of the Salmonella typhimurium glucitol phosphotransferase system.

Journal of bacteriology ·Vol. 157 ·No. 3 ·1984-03-00 ·Pages 953-5

Sarno MV, Tenn LG, Desai A, Chin AM, Grenier FC, Saier MH

Abstract

Positive selection procedures were developed for the isolation of mutants defective in components of the glucitol-specific catabolic enzyme system in Salmonella typhimurium. gutA (enzyme IIgut-negative), gutB (enzyme IIIgut-negative), and gutC (constitutive for the glucitol operon) mutants were isolated and characterized biochemically and genetically. The gene order was shown to be gutCAB.

MeSH Terms

Biological Transport Chromosome Mapping Chromosomes, Bacterial Fermentation Genes, Bacterial Mutation Operon Phosphoenolpyruvate Sugar Phosphotransferase System/genetics,metabolism Phosphorylation Salmonella typhimurium/enzymology,genetics Sorbitol/metabolism Transduction, Genetic

Chemicals

Sorbitol Phosphoenolpyruvate Sugar Phosphotransferase System phosphoenolpyruvate-sorbitol phosphotransferase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Sarno M V

Tenn L G

Desai A

Chin A M

Grenier F C

Saier M H

References (13)

13 references, click to expand

A fine structure map of the salmonella histidine operator-promoter.
Genetics. 1974 Oct;78(2):607-31 PMID: 4615040
Mutations affecting transport of the hexitols D-mannitol, D-glucitol, and galactitol in Escherichia coli K-12: isolation and mapping.
J Bacteriol. 1975 Oct;124(1):26-38 PMID: 1100602
Nature and properties of hexitol transport systems in Escherichia coli.
J Bacteriol. 1975 Oct;124(1):39-47 PMID: 1100608
Xylitol and D-arabitol toxicities due to derepressed fructose, galactitol, and sorbitol phosphotransferases of Escherichia coli.
J Bacteriol. 1977 Oct;132(1):166-73 PMID: 334721
Purification of the mannitol-specific enzyme II of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system.
J Biol Chem. 1979 Jan 25;254(2):249-52 PMID: 368051
Carbohydrate transport in bacteria.
Microbiol Rev. 1980 Sep;44(3):385-418 PMID: 6999324
The genetics of bacterial transport systems.
Annu Rev Genet. 1970;4:225-62 PMID: 4950060
Use of cloned mtl genes of Escherichia coli to introduce mtl deletion mutations into the chromosome.
J Bacteriol. 1983 Feb;153(2):685-92 PMID: 6401703
Linkage map of Escherichia coli K-12, edition 7.
Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
Mannitol-specific enzyme II of the bacterial phosphotransferase system. I. Properties of the purified permease.
J Biol Chem. 1983 Sep 10;258(17):10748-56 PMID: 6350293
Mannitol-specific enzyme II of the bacterial phosphotransferase system. III. The nucleotide sequence of the permease gene.
J Biol Chem. 1983 Sep 10;258(17):10761-7 PMID: 6309813
Sugar transport. Properties of mutant bacteria defective in proteins of the phosphoenolpyruvate: sugar phosphotransferase system.
J Biol Chem. 1976 Nov 10;251(21):6584-97 PMID: 789368
Plasmid-directed synthesis of enzymes required for D-mannitol transport and utilization in Escherichia coli.
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7336-40 PMID: 6801648

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.157.3.953-955.1984

Published

1984-03-00

Pages

953-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215355

Subset

External Links

PubMed source DOI: 10.1128/jb.157.3.953-955.1984 PMC Full Text View journal details

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Genetic evidence for glucitol-specific enzyme III, an essential phosphocarrier protein of the Salmonella typhimurium glucitol phosphotransferase system.

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