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PMID: 6361486 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunological studies of Escherichia coli mutants lacking one or two ribosomal proteins.

Molecular & general genetics : MGG ·Vol. 192 ·No. 3 ·1983-00-00 ·Pages 301-8

Dabbs ER, Hasenbank R, Kastner B, Rak KH, Wartusch B, Stöffler G

Abstract

A battery of immunological tests were used to investigate mutants which had been determined as lacking one or two ribosomal proteins on the basis of two-dimensional polyacrylamide gels. Proteins which were confirmed as missing from the ribosome in one or more mutants were large subunit proteins L1, L15, L19, L24, L27, L28, L30 and L33 and small subunit proteins S1, S9, S17 and S20. Cross-reacting material (CRM) was also absent from the post-ribosomal supernatant except in the case of protein S1. Since mutants lacking protein L11 have been previously described, any one of 13 of the 52 ribosomal proteins can be missing. None of these 13 proteins, except S1, can therefore have an indispensable role in ribosome function or assembly. In several mutants in which a protein was not missing but altered, it was present as several moieties of differing charge and size.

MeSH Terms
Bacterial Proteins/analysis,immunology Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Immune Sera Immunoenzyme Techniques Mutation Ribosomal Proteins/analysis,immunology
Chemicals
Bacterial Proteins Immune Sera Ribosomal Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Dabbs E R
Hasenbank R
Kastner B
Rak K H
Wartusch B
Stöffler G
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00392166
Published
1983-00-00
Pages
301-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00392166
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