Home LiteratureArticle Details
PMID: 6355770 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Marker rescue with plasmids bearing deletions in rpoD identifies a dispensable part of E. coli sigma factor.

Molecular & general genetics : MGG ·Vol. 191 ·No. 3 ·1983-00-00 ·Pages 492-8

Hu JC, Gross CA

Abstract

Recombinant DNA techniques allow rapid construction and characterization of tools for genetic dissection of essential genes. In this report, we describe construction of a set of deletions in rpoD, the gene which encodes the sigma subunit of E. coli RNA polymerase. We used this deletion set to identify a region of the sigma polypeptide which is dispensable for its function.

MeSH Terms
Amino Acid Sequence Chromosome Deletion Chromosome Mapping Escherichia coli/genetics Genes Genes, Bacterial Genetic Engineering Plasmids Sigma Factor/genetics,metabolism Structure-Activity Relationship Transcription Factors/genetics Transcription, Genetic
Chemicals
Sigma Factor Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hu J C
Gross C A
References (25)
25 references, click to expand
  1. Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli.
    J Mol Biol. 1978 Dec 25;126(4):847-57 PMID: 370408
  2. Mutations in the Ion gene of E. coli K12 phenotypically suppress a mutation in the sigma subunit of RNA polymerase.
    Cell. 1983 Jan;32(1):151-9 PMID: 6337720
  3. Temperature-sensitive Escherichia coli mutant producing a temperature-sensitive sigma subunit of DNA-dependent RNA polymerase.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6177-81 PMID: 366614
  4. The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12.
    Cell. 1983 Feb;32(2):335-49 PMID: 6186393
  5. The differential precipition of nucleic acids and proteins from aqueous solutions by ethanol.
    Anal Biochem. 1975 May 26;66(1):64-8 PMID: 1170771
  6. Isolation and characterization of transducing phage coding for sigma subunit of Escherichia coli RNA polymerase.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5789-93 PMID: 392509
  7. Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5453-7 PMID: 341157
  8. Cascades of Sigma factors.
    Cell. 1981 Sep;25(3):582-4 PMID: 6793235
  9. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  10. Mutation affecting thermostability of sigma subunit of Escherichia coli RNA polymerase lies near the dnaG locus at about 66 min on the E. coli genetic map.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):427-31 PMID: 343110
  11. A new mutation rpoD800, affecting the sigma subunit of E. coli RNA polymerase is allelic to two other sigma mutants.
    Mol Gen Genet. 1980 Jan;177(2):277-82 PMID: 6988671
  12. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  13. Chromosomal location of a structural gene for the RNA polymerase sigma factor in Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1831-5 PMID: 325555
  14. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.
    Biochim Biophys Acta. 1963 Aug 20;72:619-29 PMID: 14071565
  15. The selectivity of transcription.
    Annu Rev Biochem. 1974;43(0):721-75 PMID: 4605337
  16. A gene from Escherichia coli affecting the sigma subunit of RNA polymerase.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1836-40 PMID: 325556
  17. Genetic studies of the lac repressor. XI. On aspects of lac repressor structure suggested by genetic experiments.
    J Mol Biol. 1979 Jun 25;131(2):249-58 PMID: 385891
  18. On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions.
    Cell. 1982 Jun;29(2):319-28 PMID: 6288254
  19. Altered chemical properties in three mutants of E. coli RNA polymerase sigma subunit.
    Mol Gen Genet. 1979 Oct 1;175(3):251-7 PMID: 392226
  20. The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12.
    Nucleic Acids Res. 1981 Jun 25;9(12):2889-903 PMID: 6269063
  21. Multifunctional proteins.
    Annu Rev Biochem. 1976;45:143-66 PMID: 786148
  22. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
    Nucleic Acids Res. 1979 Nov 24;7(6):1541-52 PMID: 503860
  23. A procedure for isolation of spontaneous mutants with temperature sensitive of RNA and/or protein.
    Mol Gen Genet. 1977 Nov 18;156(3):233-7 PMID: 340905
  24. Transformation and preservation of competent bacterial cells by freezing.
    Methods Enzymol. 1979;68:326-31 PMID: 396437
  25. The molecular topography of RNA polymerase-promoter interaction.
    Cell. 1979 Oct;18(2):277-85 PMID: 387258
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425768
Published
1983-00-00
Pages
492-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM28575 · United States
External Links
PubMed source DOI: 10.1007/BF00425768
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2