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PMID: 6355151 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Assay of antibody to group A streptococcal carbohydrate by enzyme-linked immunosorbent assay.

Journal of clinical microbiology ·Vol. 18 ·No. 3 ·1983-09-00 ·Pages 622-7

Barrett DJ, Triggiani M, Ayoub EM

Abstract

An indirect enzyme-linked immunosorbent assay system for determination of antibody levels to the group A streptococcal cell wall carbohydrate antigen is described. Optimal conditions for antigen preparation, purification, and conjugation to poly-L-lysine for adequate adsorption to the solid phase are presented. Antibody titers of unknown sera were determined by comparison to known reference standard pool sera. A highly significant correlation (p less than 0.0001) was found between enzyme-linked immunosorbent assay antibody titers and antigen-binding capacity in a previously described radioimmunoassay. Utilizing an isotype-specific anti-immunoglobulin reagent and immunoabsorbent-purified antibody to group A streptococcal cell wall carbohydrate antigen, we were able to detect nanogram quantities of antibody by the enzyme-linked immunosorbent assay technique. This system will provide for more generalized use of group A streptococcal cell wall carbohydrate antigen antibody determinations for the study of immune responses after streptococcal infections and their complications.

MeSH Terms
Alkaline Phosphatase/immunology Antibodies, Bacterial/analysis Antigens, Bacterial/immunology Carbohydrates/analysis Enzyme-Linked Immunosorbent Assay/methods Humans Immunoglobulin G/analysis Radioimmunoassay Streptococcus pyogenes/immunology
Chemicals
Antibodies, Bacterial Antigens, Bacterial Carbohydrates Immunoglobulin G Alkaline Phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barrett D J
Triggiani M
Ayoub E M
References (18)
18 references, click to expand
  1. Persistence of streptococcal group A antibody in patients with rheumatic valvular disease.
    J Exp Med. 1968 Nov 1;128(5):1081-98 PMID: 5682941
  2. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.
    Immunochemistry. 1969 Jan;6(1):43-52 PMID: 4975324
  3. Streptococcal anti-group A precipitins in sera from patients with rheumatic arthritis and acute glomerulonephritis.
    Int Arch Allergy Appl Immunol. 1970;37(2):216-24 PMID: 4983460
  4. Precipitating antibody to group A streptococcal polysaccharide in humans.
    J Immunol. 1971 Sep;107(3):832-41 PMID: 4999097
  5. Differences in antibody response to streptococcal antigens in children with rheumatic and non-rheumatic mitral valve disease.
    Circulation. 1974 Dec;50(6):1244-51 PMID: 4430118
  6. Comparison of the titres of ASO, anti-DNase B, and antibodies against the group polysaccharide of group A streptococci in children with streptococcal infections.
    J Clin Pathol. 1974 Nov;27(11):891-6 PMID: 4216600
  7. Natural infection of man with group A streptococci. Levels; restriction in class, subclass, and type; and clonal appearance of polysaccharide-group-specific antibodies.
    Scand J Immunol. 1976;5(4):383-90 PMID: 821139
  8. Enzyme-immunoassay.
    Clin Chim Acta. 1977 Nov 15;81(1):1-40 PMID: 411611
  9. 125I protein A: applications to the quantitative determination of fluid phase and cell-bound IgG.
    J Immunol Methods. 1977;18(3-4):281-93 PMID: 591725
  10. The enzyme-linked immunosorbent assay (ELISA): a measure of antibody concentration or affinity.
    Immunochemistry. 1978 Feb;15(2):131-6 PMID: 344194
  11. ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes.
    J Immunol Methods. 1979;28(1-2):187-92 PMID: 38284
  12. The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus. I. Production and fractionation of the lytic enzymes.
    J Exp Med. 1952 Dec;96(6):555-68 PMID: 13022850
  13. Studies on the bacteriophages of hemolytic streptococci. II. Antigens released from the streptococcal cell wall by a phage-associated lysin.
    J Exp Med. 1958 Dec 1;108(6):803-21 PMID: 13598814
  14. Structure of streptococcal cell walls. I. Methylation study of C-polysaccharide.
    J Biol Chem. 1963 Feb;238:502-9 PMID: 13954323
  15. [USE OF C14-LABELLED STREPTOCOCCAL POLYSACCHARIDE FOR THE DETERMINATION OF SMALL QUANTITIES OF SPECIFIC ANTIBODIES IN EXPERIMENTAL AND HUMAN SERA].
    Rev Immunol Ther Antimicrob. 1964 Jul-Sep;28:193-204 PMID: 14257903
  16. DETECTION OF STREPTOCOCCAL GROUP-SPECIFIC ANTIBODIES IN HUMAN SERA.
    J Exp Med. 1965 Aug 1;122:195-205 PMID: 14316941
  17. The determination of glucosamine and galactosamine.
    Biochem J. 1955 Dec;61(4):586-9 PMID: 13276341
  18. Antibody titers in acute rheumatic fever.
    Circulation. 1960 Apr;21:598-614 PMID: 13842843
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.18.3.622-627.1983
Published
1983-09-00
Pages
622-7
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC270864
Subset
IM
Grants
NIAID NIH HHS · AI-17658 · United States
NHLBI NIH HHS · HL-30059 · United States
External Links
PubMed source DOI: 10.1128/jcm.18.3.622-627.1983 PMC Full Text View journal details
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