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PMID: 6352104 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Circulating antibodies to the surface antigens on colon epithelial cells in ulcerative colitis.

Clinical and experimental immunology ·Vol. 54 ·No. 1 ·1983-10-00 ·Pages 163-8

Hibi T, Aiso S, Ishikawa M, Watanabe M, Yoshida T, Kobayashi K, Asakura H, Tsuru S, Tsuchiya M

Abstract

The fluorescence activated cell sorter (FACS) was used for detecting circulating antibodies to the surface antigens on isolated colon epithelial cells (anti-colon antibodies) by indirect immunofluorescence. Anti-colon antibodies were found in the serum of 30 of 41 (73%) patients with ulcerative colitis. This incidence is much higher than one established in earlier reports by application of indirect immunofluorescence to colon tissue using the fluorescence microscope. The results suggest that FACS analysis is very useful for detecting antibodies to colon specific antigen.

MeSH Terms
Adult Animals Antibodies/analysis Antigens, Surface/immunology Cell Separation Colitis, Ulcerative/blood,immunology Colon/immunology Epithelium/immunology Female Flow Cytometry Fluorescent Antibody Technique Humans Male Rats Rats, Inbred Strains
Chemicals
Antibodies Antigens, Surface
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Hibi T
Aiso S
Ishikawa M
Watanabe M
Yoshida T
Kobayashi K
Asakura H
Tsuru S
Tsuchiya M
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17 references, click to expand
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Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1983-10-00
Pages
163-8
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1536205
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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