Home LiteratureArticle Details
PMID: 6350823 Published · ppublish English Journal Article

A novel type of bacterial transcription unit, specifying mRNA, rRNA, and tRNA.

Molecular & general genetics : MGG ·Vol. 191 ·No. 1 ·1983-00-00 ·Pages 162-4

Erdei S, Boros I, Szabó G, Venetianer P

Abstract

Previously it had been shown that a chromosomal region preceding the rrnB gene of Escherichia coli contains an 867 base pair-long open reading frame and it is co-transcribed with the rrnB gene both in vitro and in vivo. Here we report that minicells programmed by recombinant plasmids carrying this region or a deletion derivative of it, synthetise a protein of the expected size. Thus this transcription unit codes for mRNA, rRNA, and tRNA.

MeSH Terms
Escherichia coli/genetics Genes Genetic Linkage RNA, Messenger/genetics RNA, Ribosomal/genetics RNA, Transfer/genetics Transcription, Genetic
Chemicals
RNA, Messenger RNA, Ribosomal RNA, Transfer
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Erdei S
Boros I
Szabó G
Venetianer P
References (13)
13 references, click to expand
  1. In vitro transcripts from the rrn B ribosomal RNA cistron originate from two tandem promoters.
    Cell. 1979 Jan;16(1):111-21 PMID: 369703
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Dual function transcripts specifying tRNA and mRNA.
    Nature. 1981 Dec 3;294(5840):422-7 PMID: 7312036
  4. The transposon Tn1 as a probe for studying ColE1 structure and function.
    Mol Gen Genet. 1977 Mar 7;151(2):151-60 PMID: 327263
  5. Genetics of bacterial ribosomes.
    Annu Rev Genet. 1977;11:297-347 PMID: 339818
  6. Site-directed mutagenesis of ribosomal RNA. Analysis of ribosomal RNA deletion mutants using maxicells.
    J Mol Biol. 1982 Aug 15;159(3):417-39 PMID: 6187923
  7. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.
    J Mol Biol. 1981 May 15;148(2):107-27 PMID: 7028991
  8. Protein expression in E. coli minicells by recombinant plasmids.
    Cell. 1977 Mar;10(3):521-36 PMID: 403011
  9. Identification of two new promoters probably involved in the transcription of a ribosomal RNA gene of Escherichia coli.
    Biochim Biophys Acta. 1983 Mar 10;739(2):173-80 PMID: 6297582
  10. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737-41 PMID: 358200
  11. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  12. Cloning of the promoters of an Escherichia coli rRNA gene. New experimental system to study the regulation of rRNA transcription.
    Gene. 1983 May-Jun;22(2-3):191-201 PMID: 6307820
  13. Structure of the promoter region for the rrnB gene in Escherichia coli.
    Nucleic Acids Res. 1979 Dec 20;7(8):2189-97 PMID: 392468
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
Published
1983-00-00
Pages
162-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com