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PMID: 6349618 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Abnormal glucose metabolism accompanies failure of glucose to stimulate insulin release from a rat pancreatic cell line (RINm5F).

The Biochemical journal ·Vol. 212 ·No. 2 ·1983-05-15 ·Pages 439-43

Halban PA, Praz GA, Wollheim CB

Abstract

Glucose metabolism and insulin release were studied in isolated rat islets and in an insulin-producing rat cell-line (RINm5F). Intact islets displayed two components of glucose utilization, with glucose stimulation of insulin release being associated with the high-Km component (reflecting glucokinase-like activity). Glucose failed to stimulate insulin release from RINm5F cells, which only displayed a single low-Km component of glucose utilization. Only low-Km (hexokinase-like) glucose-phosphorylating activity was found for disrupted RINm5F cells. These changes in glucose metabolism may contribute towards the failure of glucose to stimulate insulin release from RINm5F cells.

MeSH Terms

Animals Cell Line Glucose/metabolism,pharmacology Insulin/immunology,metabolism Islets of Langerhans/drug effects,metabolism Male Pancreas/drug effects,metabolism Rats Rats, Inbred Strains Sonication Stimulation, Chemical

Chemicals

Insulin Glucose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Halban P A

Praz G A

Wollheim C B

References (15)

15 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2120439

Published

1983-05-15

Pages

439-43

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1152065

Subset

External Links

PubMed source DOI: 10.1042/bj2120439 PMC Full Text View journal details

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Abnormal glucose metabolism accompanies failure of glucose to stimulate insulin release from a rat pancreatic cell line (RINm5F).

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