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PMID: 634795 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence arrangement of the 16S and 26S rRNA genes in the pathogenic haemoflagellate Leishmania donovani.

Nucleic acids research ·Vol. 5 ·No. 2 ·1978-02-00 ·Pages 491-504

Leon W, Fouts DL, Manning J

Abstract

Kinetic and chemical analysis show that the haploid genome of Leishmania donovani has between 4.6 and 6.5 X 10(7) Kb pairs of DNA. Cot analysis shows that the genome contains 12% rapidly reassociating DNA, U3% middle repetitive DNA with an average reiteration frequency of 77 and 62% single copy DNA. Saturation hybridization experiments show that 0.82% of the nuclear DNA is occupied by rRNA coding sequences. The average repetition frequency of these sequences is determined to be 166. Sedimentation velocity studies indicate the two major rRNA species have sedimentation values of 26S and 16S, respectively. The arrangement of the rRNA genes and their spacer sequences on long strands of purified rDNA has been determined by the examination of the structure of rRNA:DNA hybrids prepared for electron microscopy by the gene 32-ethidium bromide technique. Long DNA strands are observed to contain several gene sets (16S + 26S). One repeat unit contains the following sequences in the order given: (a) A 16S gene of length 2.12 Kb, (b) An internal transcribed spacer (Spl) of length 1.23 Kb, which contains a short sequence that may code for a 5.8S rRNA, (C) 26S gene with a length of 4.31 Kb which contains an internal gap region of length 0.581 Ib, (d) An external spacer of average length 5.85 Kb.

MeSH Terms
Animals Chromosome Mapping Genes Kinetics Leishmania/genetics Nucleic Acid Hybridization RNA, Ribosomal/genetics Ultracentrifugation
Chemicals
RNA, Ribosomal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Leon W
Fouts D L
Manning J
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.2.491
Published
1978-02-00
Pages
491-504
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341997
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.2.491 PMC Full Text View journal details
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