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PMID: 6345805 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice.

Journal of virology ·Vol. 47 ·No. 1 ·1983-07-00 ·Pages 227-32

Merchlinsky MJ, Tattersall PJ, Leary JJ, Cotmore SF, Gardiner EM, Ward DC

Abstract

The linear single-stranded DNA genome of minute virus of mice, an autonomous parvovirus, was cloned in duplex form into the bacterial plasmid pBR322. The recombinant clones of minute virus of mice were infectious when transfected into monolayers of human 324K cells and produced virus plaques with an efficiency of about 6% that obtained with duplex replicative-form DNA purified from cells infected with minute virus of mice. Southern blot analysis of transfected cells indicated that the cloned minute virus of mice genome requires both termini to be intact for excision and replication as a linear duplex molecule.

MeSH Terms
Cells, Cultured Cloning, Molecular DNA Replication DNA, Recombinant DNA, Single-Stranded DNA, Viral/analysis Escherichia coli Genes, Viral Humans Minute Virus of Mice/genetics Parvoviridae/genetics Plasmids
Chemicals
DNA, Recombinant DNA, Single-Stranded DNA, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Merchlinsky M J
Tattersall P J
Leary J J
Cotmore S F
Gardiner E M
Ward D C
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28 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.47.1.227-232.1983
Published
1983-07-00
Pages
227-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255236
Subset
IM
Grants
NCI NIH HHS · CA-16038 · United States
NCI NIH HHS · CA-29303 · United States
NIGMS NIH HHS · GM-20124 · United States
External Links
PubMed source DOI: 10.1128/JVI.47.1.227-232.1983 PMC Full Text View journal details
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