Production of C and T bands in human mitotic chromosomes after heat treatment.

Human genetics ·Vol. 34 ·No. 2 ·1976-10-28 ·Pages 213-6

Chamla Y, Ruffié M

Abstract

A modified thermal denaturation of human chromosomes in Hanks' solution at 91 degrees C produces highly contrasted C and T bands. This improved double-banding system is a good tool in the analysis of ring chromosomes, for which R banding is often inadequate, as it is exemplified.

MeSH Terms

Chromosomes/analysis Heterochromatin/analysis Hot Temperature Humans Methods Mitosis Staining and Labeling

Chemicals

Heterochromatin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Chamla Y

Ruffié M

References (6)

6 references, click to expand

[New system of chromosome banding: the T bands (author's transl)].
Chromosoma. 1973 Apr 27;41(4):395-402 PMID: 4127019
[Factors influencing the heat denaturation of chromosomes].
Exp Cell Res. 1974 Mar 30;85(1):143-53 PMID: 4827154
Chromosome preparations of leukocytes cultured from human peripheral blood.
Exp Cell Res. 1960 Sep;20:613-6 PMID: 13772379
Production of C and T bands in human chromosomes after heat treatment at high pH and staining with "stains-all".
Humangenetik. 1974;23(4):311-4 PMID: 4138883
Localization of heterochromatin in human chromosomes.
Cytogenetics. 1971;10(2):81-6 PMID: 4106483
The mechanism of C- and G-banding of chromosomes.
Exp Cell Res. 1973 Mar 15;77(1):469-83 PMID: 4120442

Article Info

Journal

Human genetics

Abbr.

Hum Genet

ISSN

0340-6717

DOI

10.1007/BF00278891

Published

1976-10-28

Pages

213-6

Language

English

Region

Germany

NLM ID

7613873

Subset

External Links

PubMed source DOI: 10.1007/BF00278891 View journal details

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Production of C and T bands in human mitotic chromosomes after heat treatment.

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