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PMID: 6343799 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Two pathways of division inhibition in UV-irradiated E. coli.

Molecular & general genetics : MGG ·Vol. 190 ·No. 1 ·1983-00-00 ·Pages 128-32

Burton P, Holland IB

Abstract

We have investigated the mechanism of division inhibition in E. coli following UV-irradiation or nalidixic acid treatment. After UV, two separate mechanisms, both dependent upon recA, appear to block division. One mechanism is dependent upon sfiA and sfiB, is inhibited by low levels (4 micrograms/ml) of rifamycin and is expressed in tif mutants at 42 degrees C. The second mechanism is dependent on sfiA, B, is resistant to rifamycin and does not occur in cells lacking DNA replication forks. We suggest that this second mechanism is the result of the failure to terminate DNA replication in inhibited cells. Nalidixic acid inhibition of cell division also appears to involve both mechanisms but as found previously replication forks are also necessary to induce the sfi pathway.

MeSH Terms
Cell Division/drug effects,radiation effects DNA Replication Escherichia coli/cytology,genetics,radiation effects Mutation Nalidixic Acid/pharmacology Rifampin/pharmacology Ultraviolet Rays
Chemicals
Nalidixic Acid Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Burton P
Holland I B
References (21)
21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330334
Published
1983-00-00
Pages
128-32
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330334
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