Home LiteratureArticle Details
PMID: 6342691 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The structure of bovine rhodopsin.

Biophysics of structure and mechanism ·Vol. 9 ·No. 4 ·1983-00-00 ·Pages 235-44

Hargrave PA, McDowell JH, Curtis DR, Wang JK, Juszczak E, Fong SL, Rao JK, Argos P

Abstract

We have isolated 16 peptides from a cyanogen bromide digest of rhodopsin. These cyanogen bromide peptides account for the complete composition of the protein. Methionine-containing peptides from other chemical and enzymatic digests of rhodopsin have allowed us to place the cyanogen bromide peptides in order, yielding the sequence of the protein. We have completed the sequence of most of the cyanogen bromide peptides. This information, in conjunction with that from other laboratories, forms the basis for our prediction of the secondary structure of the protein and how it may be arranged in the disk membrane.

MeSH Terms
Amino Acid Sequence Animals Cattle Cyanogen Bromide Endopeptidases Molecular Weight Peptide Fragments/analysis Retinal Pigments Rhodopsin
Chemicals
Peptide Fragments Retinal Pigments Rhodopsin Endopeptidases Cyanogen Bromide
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Hargrave P A
McDowell J H
Curtis D R
Wang J K
Juszczak E
Fong S L
Rao J K
Argos P
References (10)
10 references, click to expand
  1. The carboxyl-terminal one-third of bovine rhodopsin: its structure and function.
    Vision Res. 1982;22(12):1429-38 PMID: 7182998
  2. Preparation of peptides containing any desired amino acid: methionyl peptides of bovine rhodopsin.
    Prep Biochem. 1983;13(2):83-102 PMID: 6878180
  3. Structural prediction of membrane-bound proteins.
    Eur J Biochem. 1982 Nov 15;128(2-3):565-75 PMID: 7151796
  4. Preparation of peptides from bovine rhodopsin.
    Methods Enzymol. 1982;81:251-6 PMID: 7098868
  5. Primary structure of C-terminal functional sites in ovine rhodopsin.
    Nature. 1981 Sep 24;293(5830):314-7 PMID: 7278988
  6. Partial primary structure of bacteriorhodopsin: sequencing methods for membrane proteins.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):227-31 PMID: 284335
  7. The purple membrane from Halobacterium halobium.
    Annu Rev Biophys Bioeng. 1977;6:87-109 PMID: 326156
  8. [Purification and partial sequence of a hydrophobic polypeptide from BNPS-skatole cleaved bovine rhodopsin].
    C R Seances Acad Sci D. 1980 Feb 25;290(8):567-9 PMID: 6767558
  9. The amino- and carboxyl-terminal sequence of bovine rhodopsin.
    J Supramol Struct. 1977;6(4):559-70 PMID: 592823
  10. The partial primary structure of bovine rhodopsin and its topography in the retinal rod cell disc membrane.
    Neurochem Int. 1980;1C:231-44 PMID: 20487738
Article Info
Journal
Biophysics of structure and mechanism
Abbr.
Biophys Struct Mech
ISSN
0340-1057
DOI
10.1007/BF00535659
Published
1983-00-00
Pages
235-44
Language
English
Region
Germany
NLM ID
7502020
Subset
IM
Grants
NEI NIH HHS · EY1275 · United States
NEI NIH HHS · EY2875 · United States
NIGMS NIH HHS · GM27682 · United States
External Links
PubMed source DOI: 10.1007/BF00535659
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2