Detection of Giardia lamblia by immunofluorescence.

Applied and environmental microbiology ·Vol. 45 ·No. 2 ·1983-02-00 ·Pages 698-700

Riggs JL, Dupuis KW, Nakamura K, Spath DP

Abstract

High-titer immune sera to cysts of Giardia lamblia, produced in guinea pigs, were labeled with fluorescein isothiocyanate. The resulting conjugates were used to detect G. lamblia in stool specimens by fluorescence microscopy. The sera also reacted with cysts of Chilomastix mesnili, but the two organisms could be differentiated by their size.

MeSH Terms

Animals Cross Reactions Eukaryota/immunology Feces/parasitology Fluorescent Antibody Technique Giardia/immunology,isolation & purification Humans Immune Sera Microscopy, Fluorescence

Chemicals

Immune Sera

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Riggs J L

Dupuis K W

Nakamura K

Spath D P

References (4)

4 references, click to expand

Serum antibodies and jejunal histology in giardiasis associated with malabsorption.
J Clin Pathol. 1976 Jan;29(1):30-4 PMID: 1249249
Ultrastructure of the cyst of Giardia lamblia.
Am J Trop Med Hyg. 1977 Jan;26(1):23-30 PMID: 842783
Giardiasis.
Am J Epidemiol. 1980 Jan;111(1):1-12 PMID: 6986081
An immunofluorescence test to detect serum antibodies to Giardia lamblia.
Ann Intern Med. 1980 Dec;93(6):802-5 PMID: 6255845

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.45.2.698-700.1983

Published

1983-02-00

Pages

698-700

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC242346

Subset

External Links

PubMed source DOI: 10.1128/aem.45.2.698-700.1983 PMC Full Text View journal details

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Detection of Giardia lamblia by immunofluorescence.

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