Activation and proliferation signals in mouse B cells. IV. Concanavalin A stimulates B cells to leave G0, but not to proliferate.

Immunology ·Vol. 53 ·No. 4 ·1984-12-00 ·Pages 703-11

Hawrylowicz CM, Klaus GG

Abstract

Concanavalin A (Con A), in solution, induces mouse T cells, but not B cells, to proliferate. However, lectin concentrations which were optimal for the T cell response induced purified B cells to depolarize, to enlarge, and to display increased levels of Ia antigens. Furthermore, culturing B lymphocytes with Con A for 24 hr caused the cells to synthesize DNA more rapidly in response to subsequent stimulation by lipopolysaccharide, or anti-immunoglobulin antibodies. This priming effect did not appear to require either T cells or accessory cells. It was therefore concluded that Con A activates resting B lymphocytes, i.e. stimulates them to enter the cell cycle, even though it does not induce B cells to divide.

MeSH Terms

Animals Antigen-Presenting Cells/immunology B-Lymphocytes/cytology,immunology Cell Division Concanavalin A/pharmacology DNA/biosynthesis Histocompatibility Antigens Class II/analysis Interphase Lymphocyte Activation Male Membrane Potentials Mice Mice, Inbred Strains Mitogens/pharmacology T-Lymphocytes/immunology

Chemicals

Histocompatibility Antigens Class II Mitogens Concanavalin A DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hawrylowicz C M

Klaus G G

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24 references, click to expand

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Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1984-12-00

Pages

703-11

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1454890

Subset

External Links

PubMed source PMC Full Text View journal details

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Activation and proliferation signals in mouse B cells. IV. Concanavalin A stimulates B cells to leave G0, but not to proliferate.

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