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PMID: 6333387 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Inhibition of lymphocyte proliferation by free fatty acids. II. Toxicity of stearic acid towards phytohaemagglutinin-activated T cells.

Immunology ·Vol. 53 ·No. 3 ·1984-11-00 ·Pages 507-14

Buttke TM, Cuchens MA

Abstract

Studies were performed to further characterize the effects of saturated fatty acids on murine T lymphocyte proliferation. Flow cytometry was used to show that the inhibitory effects of stearic acid (18:0) on [3H]thymidine uptake can be correlated with changes in cellular DNA content. Additional studies using flow cytometry and fluorescein diacetate as a viability stain showed that exogenous 18:0 was toxic for phytohemagglutinin (PHA)-stimulated T cells, whereas the viability of unstimulated T cells was less affected by 18:0. The inhibitory effects of 18:0 on T cell proliferation were evident as early as 4 hr after fatty acid addition and after a 10-hr exposure, the effects of 18:0 could not be reversed by washing the cells or by adding oleic acid (18:1). It is proposed that the inhibitory effects of 18:0 are dependent upon PHA-induced changes in T cell lipid metabolism.

MeSH Terms
Animals Cell Survival/drug effects DNA/analysis Female Flow Cytometry Lymphocyte Activation/drug effects Male Mice Mice, Inbred BALB C Phytohemagglutinins/pharmacology Stearic Acids/pharmacology T-Lymphocytes/analysis,drug effects,immunology
Chemicals
Phytohemagglutinins Stearic Acids stearic acid DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Buttke T M
Cuchens M A
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17 references, click to expand
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1984-11-00
Pages
507-14
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1454918
Subset
IM
Grants
NHLBI NIH HHS · HL 28468 · United States
External Links
PubMed source PMC Full Text View journal details
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