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PMID: 6332170 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification and partial biochemical characterization of normal human interleukin 1.

The Journal of experimental medicine ·Vol. 160 ·No. 3 ·1984-09-01 ·Pages 772-87

Schmidt JA

Abstract

A protocol for the rapid, efficient purification of the major charged species of human interleukin 1 (IL-1) has been developed using high performance anion exchange and size exclusion chromatography. The isolated material is pure as determined by sodium dodecyl sulfate (SDS) gradient polyacrylamide gel electrophoresis (PAGE) and analytical isoelectric focusing (IEF). The molecular weight of the purified material is 15,000 and the isoelectric point (pI) is 6.8, values that are in good agreement with those previously reported for human IL-1. 10(-10) M concentrations of the purified material give half-maximal stimulation in the thymocyte proliferation assay. Amounts of IL-1 sufficient for receptor studies and detailed biochemical analysis can now be produced on a regular basis.

MeSH Terms
Animals Chromatography, DEAE-Cellulose Chromatography, High Pressure Liquid Culture Media Electrophoresis, Polyacrylamide Gel Humans Interleukin-1/biosynthesis,isolation & purification,physiology Isoelectric Focusing Lymphocyte Activation Mice Molecular Weight T-Lymphocytes/immunology,metabolism
Chemicals
Culture Media Interleukin-1
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Schmidt J A
References (38)
38 references, click to expand
  1. Potentiation of the T-lymphocyte response to mitogens. I. The responding cell.
    J Exp Med. 1972 Jul 1;136(1):128-42 PMID: 5033417
  2. Effects of carrier ampholyte contamination on the biological and biochemical properties of streptococcal pyrogenic exotoxin type C.
    Infect Immun. 1983 Jan;39(1):311-4 PMID: 6337098
  3. Membrane receptors and hormone action.
    Adv Protein Chem. 1976;30:251-451 PMID: 180780
  4. Purification and properties of human lymphocyte activating factor (LAF).
    J Immunol. 1977 May;118(5):1631-8 PMID: 300751
  5. Association of a low molecular weight helper factor(s) with thymocyte proliferative activity.
    J Immunol. 1977 Jul;119(1):55-60 PMID: 301539
  6. Secretion of mediators following T lymphocyte-macrophage interaction is regulated by the major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3542-6 PMID: 410022
  7. Partial purification of human lymphocyte-activating factor (LAF) by ultrafiltration and electrophoretic techniques.
    J Immunol. 1977 Dec;119(6):2019-23 PMID: 915290
  8. Human leukocytic pyrogen: purification and development of a radioimmunoassay.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4624-7 PMID: 22079
  9. Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
    Anal Biochem. 1978 May;86(1):142-6 PMID: 655375
  10. Interleukin 1 stimulation of collagenase production by cultured fibroblasts.
    J Exp Med. 1983 Feb 1;157(2):801-6 PMID: 6296266
  11. Phorbol diester receptor copurifies with protein kinase C.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):36-40 PMID: 6296873
  12. Role of interleukin 1 in anti-immunoglobulin-induced B cell proliferation.
    J Exp Med. 1983 May 1;157(5):1529-43 PMID: 6602200
  13. A rapid, large scale purification procedure for gibbon interleukin 2.
    J Immunol. 1983 Aug;131(2):810-5 PMID: 6602847
  14. Interleukin 1 production by a human acute monocytic leukemia cell line.
    Cell Immunol. 1983 Sep;80(2):223-9 PMID: 6309415
  15. Preparation of goat antibodies against interleukin 1: use of an immunoadsorbent to purify interleukin 1.
    J Immunol. 1983 Oct;131(4):1834-7 PMID: 6352805
  16. High-performance liquid chromatography of biopolymers.
    Science. 1983 Oct 21;222(4621):245-52 PMID: 6353575
  17. Silica-stimulated monocytes release fibroblast proliferation factors identical to interleukin 1. A potential role for interleukin 1 in the pathogenesis of silicosis.
    J Clin Invest. 1984 May;73(5):1462-72 PMID: 6325504
  18. Isoelectric points of proteins: a table.
    Anal Biochem. 1978 Jun 1;86(2):620-47 PMID: 26290
  19. Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line, P388D1. I. Enhancement of LAF production by activated T lymphocytes.
    J Immunol. 1978 May;120(5):1497-503 PMID: 149171
  20. T cell growth factor: parameters of production and a quantitative microassay for activity.
    J Immunol. 1978 Jun;120(6):2027-32 PMID: 307029
  21. Lymphocyte-activating factor. I. Generation and physicochemical characterization.
    J Immunol. 1978 Oct;121(4):1446-52 PMID: 308965
  22. Human leukocyte interferon: production, purification to homogeneity, and initial characterization.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):640-4 PMID: 284389
  23. Physicochemical characterization of lymphocyte-activating factor (LAF).
    J Immunol. 1979 Jun;122(6):2167-72 PMID: 312860
  24. Signal requirements for T lymphocyte activation. I. Replacement of macrophage function with phorbol myristic acetate.
    J Immunol. 1979 Oct;123(4):1749-54 PMID: 314470
  25. Studies on T lymphocyte activation II. The target cells for concanavalin A-induced growth factors.
    Eur J Immunol. 1979 Aug;9(8):587-92 PMID: 159185
  26. Ligand-activated T cell growth factor-induced proliferation: absorption of T cell growth factor by activated T cells.
    J Immunol. 1979 Dec;123(6):2704-8 PMID: 315430
  27. Endogenous pyrogens made by rabbit peritoneal exudate cells are identical with lymphocyte-activating factors made by rabbit alveolar macrophages.
    J Immunol. 1980 May;124(5):2498-501 PMID: 6965973
  28. Mitogenic and inhibitory effects of carrier ampholytes on quiescent and stimulated human diploid lung fibroblasts.
    Anal Biochem. 1979 Sep 15;98(1):27-35 PMID: 44437
  29. The functional relationship of the interleukins.
    J Exp Med. 1980 Jun 1;151(6):1551-6 PMID: 6770028
  30. The role of subcellular factors in pulmonary immune function: physicochemical characterization of two distinct species of lymphocyte-activating factor produced by rabbit alveolar macrophages.
    J Immunol. 1981 Apr;126(4):1534-41 PMID: 6782162
  31. Purification of human interleukin 1.
    J Supramol Struct. 1980;13(4):457-66 PMID: 6785531
  32. Stimulation of rheumatoid synovial cell collagenase and prostaglandin production by partially purified lymphocyte-activating factor (interleukin 1).
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2474-7 PMID: 6264478
  33. The role of macrophages in the acute-phase response: SAA inducer is closely related to lymphocyte activating factor and endogenous pyrogen.
    Cell Immunol. 1981 Sep 1;63(1):164-76 PMID: 6791836
  34. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
    Anal Biochem. 1981 Nov 1;117(2):307-10 PMID: 6172996
  35. Murine epidermal cell-derived thymocyte-activating factor resembles murine interleukin 1.
    J Immunol. 1982 May;128(5):2147-52 PMID: 6801132
  36. Interleukin 1, a potential regulator of fibroblast proliferation.
    J Immunol. 1982 May;128(5):2177-82 PMID: 6460819
  37. Purification by reverse-phase high-performance liquid chromatography of an epidermal growth factor-dependent transforming growth factor.
    Anal Biochem. 1982 Sep 1;125(1):217-24 PMID: 6983313
  38. Lack of interaction between hormonal proteins and synthetic carrier ampholytes (Ampholine).
    Anal Biochem. 1975 Apr;64(2):530-6 PMID: 1130665
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.160.3.772
Published
1984-09-01
Pages
772-87
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187399
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.160.3.772 PMC Full Text View journal details
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