A colorimetric method for DNA hybridization.

Nucleic acids research ·Vol. 12 ·No. 8 ·1984-04-25 ·Pages 3435-44

Renz M, Kurz C

Abstract

A general method has been developed which allows crosslinks to be produced between proteins and single-stranded DNA. Such single-stranded DNA protein complexes have been tested for blot hybridization using two colorimetrically detectable enzymes, namely peroxidase and alkaline phosphatase, as the protein moiety of the probe. After hybridization and incubation with a substrate solution sequences complementary to the probe can be visualized directly without the need of tedious cytochemical sandwich methods. This procedure will detect target sequences, a few kilobases long, in the 1- to 5-pg range.

MeSH Terms

Animals Base Sequence Colorimetry/methods DNA Restriction Enzymes DNA, Circular DNA, Single-Stranded/analysis Drosophila Drosophila melanogaster Horseradish Peroxidase Indicators and Reagents Nucleic Acid Hybridization

Chemicals

DNA, Circular DNA, Single-Stranded Indicators and Reagents Horseradish Peroxidase DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Renz M

Kurz C

References (12)

12 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.8.3435

Published

1984-04-25

Pages

3435-44

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318760

Subset

External Links

PubMed source DOI: 10.1093/nar/12.8.3435 PMC Full Text View journal details

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A colorimetric method for DNA hybridization.

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