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PMID: 6328011 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the env gene and long terminal repeat of molecularly cloned Friend mink cell focus-inducing virus DNA.

Journal of virology ·Vol. 50 ·No. 3 ·1984-06-00 ·Pages 813-21

Adachi A, Sakai K, Kitamura N, Nakanishi S, Niwa O, Matsuyama M, Ishimoto A

Abstract

The highly oncogenic erythroleukemia-inducing Friend mink cell focus-inducing (MCF) virus was molecularly cloned in phage lambda gtWES.lambda B, and the DNA sequences of the env gene and the long terminal repeat were determined. The nucleotide sequences of Friend MCF virus and Friend spleen focus-forming virus were quite homologous, supporting the hypothesis that Friend spleen focus-forming virus might be generated via Friend MCF virus from an ecotropic Friend virus mainly by some deletions. Despite their different pathogenicity, the nucleotide sequences of the env gene of Friend MCF virus and Moloney MCF virus were quite homologous, suggesting that the putative parent sequence for the generation of both MCF viruses and the recombinational mechanism for their generation might be the same. We compare the amino acid sequences in lymphoid leukemia-inducing ecotropic Moloney virus and Moloney MCF virus, and erythroblastic leukemia-inducing ecotropic Friend virus, Friend-MCF virus, and Friend spleen focus-forming virus. The Friend MCF virus long terminal repeat was found to be 550 base pairs long. This contained two copies of the 39-base-pair tandem repeat, whereas the spleen focus-forming virus genome contained a single copy of the same sequence.

MeSH Terms
Animals Base Sequence Cell Line Cloning, Molecular DNA Restriction Enzymes DNA, Viral/genetics Friend murine leukemia virus/genetics Genes Genes, Viral Lung Mink Viral Envelope Proteins/genetics
Chemicals
DNA, Viral Viral Envelope Proteins DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Adachi A
Sakai K
Kitamura N
Nakanishi S
Niwa O
Matsuyama M
Ishimoto A
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45 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.3.813-821.1984
Published
1984-06-00
Pages
813-21
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255741
Subset
IM
Databases
GENBANK
K01384, K01385, M16688
External Links
PubMed source DOI: 10.1128/JVI.50.3.813-821.1984 PMC Full Text View journal details
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