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PMID: 6327619 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic and biochemical characterization of the galactose gene cluster in Kluyveromyces lactis.

Journal of bacteriology ·Vol. 158 ·No. 2 ·1984-05-00 ·Pages 705-12

Riley MI, Dickson RC

Abstract

We isolated and identified mutant strains of Kluyveromyces lactis that are defective for the Leloir pathway enzymes galactokinase, transferase, and epimerase, and we termed these loci GAL1 , GAL7 , and GAL10 , respectively. Genetic data indicate that these three genes are tightly linked, having an apparent order of GAL7 - GAL10 - GAL1 . This same gene order has been observed in Saccharomyces cerevisiae. Strains harboring gal7 mutations have elevated levels of beta-galactosidase, coded by an unlinked gene, galactokinase, and epimerase activities under uninduced conditions. We investigated the genetic basis of this constitutive gene expression and found no recombinants between the constitutive and Gal- phenotypes among 76 tetrads, suggesting that either GAL7 or a tightly linked gene codes for a regulatory function. This is the second gene that has been shown to specifically coregulate expression of the genes coding for beta-galactosidase and the Leloir pathway enzymes.

MeSH Terms
Ascomycota/genetics Carbohydrate Epimerases/genetics Galactokinase/genetics Galactose/metabolism Genes, Fungal Genes, Regulator Genetic Linkage Hot Temperature Mutation Nucleotidyltransferases/genetics Recombination, Genetic Saccharomycetales/enzymology,genetics UDPglucose 4-Epimerase UTP-Hexose-1-Phosphate Uridylyltransferase/genetics
Chemicals
Galactokinase Nucleotidyltransferases UTP-Hexose-1-Phosphate Uridylyltransferase Carbohydrate Epimerases UDPglucose 4-Epimerase galactose epimerase Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Riley M I
Dickson R C
References (33)
33 references, click to expand
  1. Evidence for chain termination by super-suppressible mutants in yeast.
    Genetics. 1968 Dec;60(4):719-33 PMID: 5732414
  2. lac Repressor-operator interaction. VI. The natural inducer of the lac operon.
    J Mol Biol. 1972 Aug 28;69(3):397-408 PMID: 4562709
  3. Lac4 is the structural gene for beta-galactosidase in Kluyveromyces lactis.
    Genetics. 1981 Aug;98(4):729-45 PMID: 6800877
  4. Recessive mutations conferring resistance to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae.
    J Bacteriol. 1983 Mar;153(3):1405-14 PMID: 6337998
  5. SEPARATION OF SUGAR NUCLEOTIDES, PHOSPHORIC ESTERS AND FREE SUGARS BY PAPER CHROMATOGRAPHY WITH SOLVENTS CONTAINING BORATES OF ORGANIC BASES.
    J Chromatogr. 1965 May;18:342-8 PMID: 14318660
  6. Deletion analysis of the Saccharomyces GAL gene cluster. Transcription from three promoters.
    J Mol Biol. 1981 Oct 25;152(2):317-34 PMID: 7035681
  7. Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae.
    Mol Cell Biol. 1981 Feb;1(2):83-93 PMID: 6765598
  8. Interference with growth of certain Escherichia coli mutants by galactose.
    Biochim Biophys Acta. 1958 Nov;30(2):298-302 PMID: 13607446
  9. Characterization of lactose transport in Kluyveromyces lactis.
    J Bacteriol. 1983 Jun;154(3):1245-51 PMID: 6853445
  10. The enzymes of the galactose cluster in Saccharomyces cerevisiae. Purification and characterization of galactose-1-phosphate uridylyltransferase.
    J Biol Chem. 1979 Nov 10;254(21):10707-9 PMID: 387754
  11. A cluster of three genes responsible for allantoin degradation in Saccharomyces cerevisiae.
    Genetics. 1979 Jun;92(2):383-96 PMID: 385448
  12. Detection of phosphate esters on chromatograms: an improved reagent.
    Anal Biochem. 1981 Oct;117(1):81-3 PMID: 7316201
  13. Analytical methods for yeasts.
    Methods Cell Biol. 1975;12:111-47 PMID: 172763
  14. Reagent for Differentiation of 1,4- and 1,6-Linked Glucosaccharides.
    Science. 1956 Mar 30;123(3196):543-4 PMID: 17730453
  15. Bipartite structure of the ade3 locus of Saccharomyces cerevisiae.
    Genetics. 1977 Feb;85(2):209-23 PMID: 324867
  16. Isolation and preliminary characterization of the GAL4 gene, a positive regulator of transcription in yeast.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6827-31 PMID: 6294656
  17. Galactose regulation in Saccharomyces cerevisiae. The enzymes encoded by the GAL7, 10, 1 cluster are co-ordinately controlled and separately translated.
    J Mol Biol. 1979 Jun 15;131(1):41-53 PMID: 385888
  18. Formyl-methenyl-methylenetetrahydrofolate synthetase (combined); correlation of enzymic activities with limited proteolytic degradation of the protein from yeast.
    Biochem Biophys Res Commun. 1977 Jul 11;77(1):147-54 PMID: 329838
  19. The organization and transcription of the galactose gene cluster of Saccharomyces.
    J Mol Biol. 1981 Oct 25;152(2):285-315 PMID: 6276569
  20. Biochemical Mutants in the Smut Fungus Ustilago Maydis.
    Genetics. 1949 Sep;34(5):607-26 PMID: 17247336
  21. Mutations affecting synthesis of beta-galactosidase activity in the yeast Kluyveromyces lactis.
    Genetics. 1980 Aug;95(4):877-90 PMID: 6781984
  22. ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES.
    Genetics. 1964 May;49:837-44 PMID: 14158615
  23. Galactose-sensitive mutants of Salmonella.
    Nature. 1959 Oct 10;184(Suppl 15):1168-9 PMID: 13825592
  24. Induction of the allantoin degradative enzymes in Saccharomyces cerevisiae by the last intermediate of the pathway.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2340-4 PMID: 4599622
  25. Structural analysis of the dur loci in S. cerevisiae: two domains of a single multifunctional gene.
    Genetics. 1980 Mar;94(3):555-80 PMID: 6105114
  26. HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, II. GALACTOSE-INDUCED SENSITIVITY.
    Proc Natl Acad Sci U S A. 1959 Dec;45(12):1786-91 PMID: 16590576
  27. Genetic order of the galactose structural genes in Saccharomyces cerevisiae.
    J Bacteriol. 1971 Oct;108(1):179-83 PMID: 5122803
  28. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  29. Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6971-5 PMID: 6294669
  30. Induction of galactokinase in Saccharomyces cerevisiae: kinetics of induction and glucose effects.
    J Bacteriol. 1972 Aug;111(2):308-15 PMID: 4559724
  31. Genetic regulation: yeast mutants constitutive for beta-galactosidase activity have an increased level of beta-galactosidase messenger ribonucleic acid.
    Mol Cell Biol. 1981 Nov;1(11):1048-56 PMID: 6810093
  32. Physiological studies of beta-galactosidase induction in Kluyveromyces lactis.
    J Bacteriol. 1980 Jun;142(3):777-85 PMID: 6769910
  33. Genetic co-regulation of galactose and melibiose utilization in Saccharomyces.
    J Bacteriol. 1976 Jan;125(1):33-41 PMID: 1245460
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.2.705-712.1984
Published
1984-05-00
Pages
705-12
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215487
Subset
IM
Grants
NIGMS NIH HHS · GM 22749 · United States
External Links
PubMed source DOI: 10.1128/jb.158.2.705-712.1984 PMC Full Text View journal details
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