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PMID: 6324191 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Assembly in vitro of a spanning membrane protein of the endoplasmic reticulum: the E1 glycoprotein of coronavirus mouse hepatitis virus A59.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 5 ·1984-03-00 ·Pages 1421-5

Rottier P, Brandenburg D, Armstrong J, van der Zeijst B, Warren G

Abstract

The E1 glycoprotein of coronavirus mouse hepatitis virus A59 was synthesized in vitro by translation of viral mRNA in the presence of dog pancreatic microsomes. Its disposition in the membrane was investigated by digestion with proteases and by selective NH2-terminal labeling. The protein spans the membrane, but only small portions from the NH2 and COOH terminus are exposed respectively in the lumenal and cytoplasmic domains; the bulk of the molecule is apparently buried in the membrane. The protein lacks a cleavable leader sequence and does not acquire its characteristic O-linked oligosaccharides in rough microsomes. It may enter the membrane at any stage during synthesis of the first 150 amino acid residues. These unusual features of the protein might help to explain why it is not transported to the cell surface in vivo but remains in intracellular membranes, causing the virus to bud there.

MeSH Terms
Animals Dogs Electrophoresis, Polyacrylamide Gel Endoplasmic Reticulum/metabolism Lipid Bilayers Membrane Proteins/genetics Microsomes/metabolism Murine hepatitis virus/genetics Pancreas/metabolism Protein Biosynthesis RNA, Messenger/genetics Viral Proteins/genetics Virion/genetics
Chemicals
Lipid Bilayers Membrane Proteins RNA, Messenger Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rottier P
Brandenburg D
Armstrong J
van der Zeijst B
Warren G
References (40)
40 references, click to expand
  1. Initiation of haemoglobin synthesis by methionyl-tRNA.
    Nature. 1970 Aug 29;227(5261):913-8 PMID: 4915406
  2. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes.
    J Cell Biol. 1970 Apr;45(1):146-57 PMID: 5458993
  3. Inhibition of peptide initiation on reticulocyte ribosomes by edeine.
    Eur J Biochem. 1971 Jul 15;21(1):31-41 PMID: 5568674
  4. Preparation and analysis of L-( 35 S)methionine labeled transfer ribonucleic acids from rabbit liver.
    Anal Biochem. 1972 Jul;48(1):202-16 PMID: 4557421
  5. Intracellular aspects of the process of protein synthesis.
    Science. 1975 Aug 1;189(4200):347-58 PMID: 1096303
  6. Three-dimensional model of purple membrane obtained by electron microscopy.
    Nature. 1975 Sep 4;257(5521):28-32 PMID: 1161000
  7. Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.
    J Cell Biol. 1975 Dec;67(3):852-62 PMID: 811672
  8. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  9. I. Structural proteins: effects of preparative conditions on the migration of protein in polyacrylamide gels.
    Virology. 1977 Apr;77(2):637-49 PMID: 855186
  10. The dynamics of maternal poly(A)-containing mRNA in fertilized sea urchin eggs.
    Cell. 1977 Jul;11(3):673-81 PMID: 560258
  11. Membrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a transmembrane protein.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3278-82 PMID: 198778
  12. Synchronised transmembrane insertion and glycosylation of a nascent membrane protein.
    Nature. 1977 Oct 27;269(5631):775-80 PMID: 200844
  13. Ovalbumin: a secreted protein without a transient hydrophobic leader sequence.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):94-8 PMID: 272676
  14. Assembly of the Semliki Forest virus membrane glycoproteins in the membrane of the endoplasmic reticulum in vitro.
    J Mol Biol. 1978 Oct 5;124(4):587-600 PMID: 712848
  15. The band 3 protein of the human red cell membrane: a review.
    J Supramol Struct. 1978;8(3):311-24 PMID: 364194
  16. Membrane biogenesis. In vitro cleavage, core glycosylation, and integration into microsomal membranes of sindbis virus glycoproteins.
    J Cell Biol. 1979 Jan;80(1):219-24 PMID: 422651
  17. The structural basis of the functioning of bacteriorhodopsin: an overview.
    FEBS Lett. 1979 Apr 15;100(2):219-24 PMID: 378693
  18. Messenger RNA of opsin from bovine retina: isolation and partial sequence of the in vitro translation product.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2654-8 PMID: 288054
  19. In vitro synthesis, glycosylation, and membrane insertion of influenza virus haemagglutinin.
    Virology. 1979 Jun;95(2):343-50 PMID: 462798
  20. Chicken ovalbumin contains an internal signal sequence.
    Nature. 1979 Sep 13;281(5727):117-21 PMID: 471058
  21. Synthesis and insertion of cytochrome P-450 into endoplasmic reticulum membranes.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):965-9 PMID: 6767247
  22. Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid.
    J Virol. 1980 Jan;33(1):449-62 PMID: 6245243
  23. Phase separation of integral membrane proteins in Triton X-114 solution.
    J Biol Chem. 1981 Feb 25;256(4):1604-7 PMID: 6257680
  24. Isolation and identification of virus-specific mRNAs in cells infected with mouse hepatitis virus (MHV-A59).
    Virology. 1981 Jan 30;108(2):424-34 PMID: 6258295
  25. Passage of an integral membrane protein, the vesicular stomatitis virus glycoprotein, through the Golgi apparatus en route to the plasma membrane.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1746-50 PMID: 6262824
  26. Translation of three mouse hepatitis virus strain A59 subgenomic RNAs in Xenopus laevis oocytes.
    J Virol. 1981 Apr;38(1):20-6 PMID: 6264100
  27. Tunicamycin resistant glycosylation of coronavirus glycoprotein: demonstration of a novel type of viral glycoprotein.
    Virology. 1981 Dec;115(2):334-44 PMID: 7314449
  28. Viral protein synthesis in mouse hepatitis virus strain A59-infected cells: effect of tunicamycin.
    J Virol. 1981 Nov;40(2):350-7 PMID: 6275093
  29. Passage of viral membrane proteins through the Golgi complex.
    J Mol Biol. 1981 Nov 15;152(4):663-98 PMID: 7038131
  30. The erythrocyte anion transport protein is contranslationally inserted into microsomes.
    Cell. 1982 Jan;28(1):23-31 PMID: 7066984
  31. Cell tropism and expression of mouse hepatitis viruses (MHV) in mouse spinal cord cultures.
    Virology. 1982 Jun;119(2):317-31 PMID: 6281976
  32. Coronavirus glycoprotein E1, a new type of viral glycoprotein.
    J Mol Biol. 1981 Dec 25;153(4):993-1010 PMID: 7343686
  33. A membrane glycoprotein that accumulates intracellularly: cellular processing of the large glycoprotein of LaCrosse virus.
    Cell. 1982 Apr;28(4):821-9 PMID: 6284375
  34. Morphogenesis of sandfly viruses (Bunyaviridae family).
    Virology. 1982 Aug;121(1):61-81 PMID: 6287722
  35. The signal sequence of ovalbumin is located near the NH2 terminus.
    J Biol Chem. 1982 Oct 25;257(20):12245-51 PMID: 6749856
  36. Uukuniemi virus maturation: accumulation of virus particles and viral antigens in the Golgi complex.
    Mol Cell Biol. 1982 Nov;2(11):1444-58 PMID: 6891745
  37. O-linked oligosaccharides are acquired by herpes simplex virus glycoproteins in the Golgi apparatus.
    Cell. 1983 Mar;32(3):987-97 PMID: 6299584
  38. Structural homology of Torpedo californica acetylcholine receptor subunits.
    Nature. 1983 Apr 7;302(5908):528-32 PMID: 6188060
  39. Persistent infection of some standard cell lines by lymphocytic choriomeningitis virus: transmission of infection by an intracellular agent.
    J Virol. 1983 Oct;48(1):249-61 PMID: 6193287
  40. Post-translational glycosylation of coronavirus glycoprotein E1: inhibition by monensin.
    EMBO J. 1982;1(12):1499-504 PMID: 6327272
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.5.1421
Published
1984-03-00
Pages
1421-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344847
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.5.1421 PMC Full Text View journal details
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