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PMID: 6323738 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell-mediated cytotoxicity against targets bearing Sendai virus glycoproteins in the absence of viral infection.

Journal of virology ·Vol. 50 ·No. 2 ·1984-05-00 ·Pages 380-6

Abidi TF, Flanagan TD

Abstract

The glycoproteins HN and F and the lipids were solubilized from Sendai virus envelopes by using the nonionic detergent beta-D-octylglucoside. When beta-D-octylglucoside was removed by dialysis, the glycoproteins and lipids reassociated to form vesicles. These vesicles displayed hemagglutinating, neuraminidase, and hemolysin activities comparable to those expressed by the intact virus. The vesicles were used as carriers to transfer the glycoproteins to the surface of P815 cells. The recipient cells were tested for the acquisition of the glycoproteins by demonstration of surface neuraminidase, hemadsorption activity, and antigens. The modified cells were used as targets for natural cell-mediated lysis and were found to be sensitive.

MeSH Terms
Animals Antigens, Viral/immunology Cell Line Chick Embryo Electrophoresis, Disc Fluorescent Antibody Technique Glycoproteins/immunology Hemolysis Humans Immunity, Cellular Killer Cells, Natural/immunology Lipids/immunology Mice Mice, Nude Parainfluenza Virus 1, Human/immunology Plasmacytoma/immunology Viral Envelope Proteins/immunology
Chemicals
Antigens, Viral Glycoproteins Lipids Viral Envelope Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Abidi T F
Flanagan T D
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.2.380-386.1984
Published
1984-05-00
Pages
380-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255631
Subset
IM
Grants
NIAID NIH HHS · T32AI07088 · United States
External Links
PubMed source DOI: 10.1128/JVI.50.2.380-386.1984 PMC Full Text View journal details
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